Effect of argon plasma pre-treatment of healing abutments on peri-implant microbiome and soft tissue integration: a proof-of-concept randomized study.
Argon plasma
Biofilms
Dental implants
Healing abutments
Microbiome
Soft tissues
Journal
BMC oral health
ISSN: 1472-6831
Titre abrégé: BMC Oral Health
Pays: England
ID NLM: 101088684
Informations de publication
Date de publication:
17 01 2023
17 01 2023
Historique:
received:
06
10
2022
accepted:
10
01
2023
entrez:
17
1
2023
pubmed:
18
1
2023
medline:
20
1
2023
Statut:
epublish
Résumé
Biofilm-free implant surface is ultimate prerequisite for successful soft and bone tissue integration. Objective of the study was to estimate the effects of argon plasma healing abutment pre-treatment (PT) on peri-implant soft-tissue phenotype (PiSP), inflammation, plaque accumulation and the microbiome (PiM) between non-treated (NPT) and treated (PT) abutments following 3-months healing period. The hypothesis was that cell-conductive and antimicrobial properties of PT would yield optimal conditions for soft tissue integration. Two months following second-phase surgery, microbiological and clinical parameters were assessed around thirty-six healing abutments with two types of microtopography, smooth surface (MACHINED) and ultrathin threaded microsurface (ROUGH). A two level randomization schema was used to achieve equal distribution and abutments were randomly divided into rough and machined groups, and then divided into PT and NPT groups. PiM was assessed using next-generation DNA sequencing. PiM bacterial composition was highly diverse already two months post-implantation, consisting of key-stone pathogens, early and late colonizers, while the mycobiome was less diverse. PT was associated with lower plaque accumulation and inflammation without significant impact on PiSP, while in NPT clinical parameters were increased and associated with periopathogens. NPT mostly harbored late colonizers, while PT exerted higher abundance of early colonizers suggesting less advanced plaque formation. Interaction analysis in PT demonstrated S. mitis co-occurrence with pro-healthy Rothia dentocariosa and co-exclusion with Parvimonas micra, Porphyromonas endodontalis and Prevotella oris. PiSP parameters were generally similar between the groups, but significant association between PiM and keratinized mucosa width was observed in both groups, with remarkably more expressed diversity in NPT compared to PT. PT resulted in significantly lower BOP and PI around rough and machined abutments, respectively, without specific effect on PiM and PiSP. PT contributed to significantly the less advanced biofilm accumulation and inflammation without specific effects on PiSP.
Identifiants
pubmed: 36650477
doi: 10.1186/s12903-023-02729-1
pii: 10.1186/s12903-023-02729-1
pmc: PMC9843976
doi:
Substances chimiques
Argon
67XQY1V3KH
Dental Implants
0
Plasma Gases
0
Titanium
D1JT611TNE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
27Informations de copyright
© 2023. The Author(s).
Références
Periodontol 2000. 2015 Jun;68(1):182-216
pubmed: 25867987
J Clin Periodontol. 1995 Mar;22(3):255-61
pubmed: 7790533
Acta Biomater. 2021 Sep 15;132:325-344
pubmed: 33857691
Clin Oral Implants Res. 2014 Mar;25(3):328-336
pubmed: 23210704
Clin Oral Implants Res. 2018 Mar;29 Suppl 15:32-49
pubmed: 29498129
J Biomed Mater Res A. 2005 Jul 1;74(1):49-58
pubmed: 15924300
Dent Mater. 2021 Feb;37(2):359-369
pubmed: 33358017
Mol Oral Microbiol. 2014 Dec;29(6):248-57
pubmed: 24976068
J Clin Periodontol. 2019 Jun;46 Suppl 21:82-91
pubmed: 31215114
PLoS One. 2012;7(8):e42539
pubmed: 22880025
J Craniomaxillofac Surg. 2008 Dec;36(8):477-80
pubmed: 18774302
Int Wound J. 2017 Feb;14(1):89-96
pubmed: 26688157
PLoS One. 2014 Mar 10;9(3):e90899
pubmed: 24614173
Int J Oral Maxillofac Implants. 2016 Mar-Apr;31(2):359-68
pubmed: 26478978
Clin Oral Implants Res. 2003 Jun;14(3):263-8
pubmed: 12755775
J Clin Periodontol. 2015 Apr;42 Suppl 16:S152-7
pubmed: 25626479
J Clin Periodontol. 2017 Dec;44(12):1274-1284
pubmed: 28766745
J Periodontol. 2020 Mar;91(3):283-288
pubmed: 32027021
Periodontol 2000. 2014 Oct;66(1):255-73
pubmed: 25123773
J Biophotonics. 2017 May;10(5):718-726
pubmed: 27539641
Biomaterials. 2013 Nov;34(33):8018-29
pubmed: 23932292
J Clin Periodontol. 2018 Jun;45 Suppl 20:S286-S291
pubmed: 29926491
Mol Oral Microbiol. 2018 Oct;33(5):388-395
pubmed: 30007055
Dent Mater. 2014 Jul;30(7):716-27
pubmed: 24793200
Dent Mater. 2020 Sep;36(9):e279-e291
pubmed: 32591158
Arch Oral Biol. 2019 Aug;104:7-12
pubmed: 31153099
Clin Oral Implants Res. 2017 May;28(5):512-519
pubmed: 27079924
Periodontol 2000. 2018 Feb;76(1):97-108
pubmed: 29193310
Arch Oral Biol. 2014 Jan;59(1):66-72
pubmed: 24209597
Nanomaterials (Basel). 2020 Jul 31;10(8):
pubmed: 32751895
Acta Biomater. 2016 Apr 1;34:41-52
pubmed: 26196081
J Clin Med. 2020 Aug 31;9(9):
pubmed: 32878146
Infect Immun. 2014 May;82(5):1968-81
pubmed: 24566629
Nat Immunol. 2020 Sep;21(9):974-982
pubmed: 32747813
Biomaterials. 2015 Jun;52:327-34
pubmed: 25818439
Clin Oral Investig. 2020 Mar;24(3):1113-1124
pubmed: 31955269
Int J Mol Sci. 2020 Aug 22;21(17):
pubmed: 32842709
J Clin Periodontol. 2015 Apr;42 Suppl 16:S1-4
pubmed: 25683242
Clin Oral Investig. 2018 May;22(4):1805-1816
pubmed: 29218422
Adv Exp Med Biol. 2015;830:69-84
pubmed: 25366221
Acta Biomater. 2021 Sep 15;132:1-3
pubmed: 34503734
J Dent Res. 2021 Jan;100(1):21-28
pubmed: 32783779
J Biomed Mater Res A. 2012 Jul;100(7):1901-6
pubmed: 22492543
Med Oral Patol Oral Cir Bucal. 2016 Jan 01;21(1):e118-21
pubmed: 26595834
J Clin Periodontol. 2017 Jun;44(6):672-680
pubmed: 28303583
J Biomed Mater Res A. 2005 Jun 1;73(3):264-74
pubmed: 15789411
Int J Oral Maxillofac Implants. 2013 Jul-Aug;28(4):957-62
pubmed: 23869352
Nat Rev Microbiol. 2012 Oct;10(10):717-25
pubmed: 22941505
Mol Oral Microbiol. 2015 Oct;30(5):411-24
pubmed: 25965671
J Dent Res. 2016 Jan;95(1):43-9
pubmed: 26701919
J Periodontol. 2019 May;90(5):445-453
pubmed: 30461016
Clin Oral Implants Res. 2012 Sep;23(9):1045-54
pubmed: 22092339
Int J Nanomedicine. 2018 Oct 08;13:6123-6141
pubmed: 30349241
Int J Oral Maxillofac Implants. 2018 Nov/Dec;33(6):1247-1254
pubmed: 30427955
J Periodontol. 2017 Aug;88(8):752-761
pubmed: 28440740
J Dent Res. 2021 Jan;100(1):74-81
pubmed: 32853527
Dent Mater. 2020 Jun;36(6):e194-e205
pubmed: 32360041
Ann Microbiol. 2015;65(3):1537-1546
pubmed: 26273240
Genome Biol. 2011 Jun 24;12(6):R60
pubmed: 21702898
Periodontol 2000. 2019 Oct;81(1):29-40
pubmed: 31407437
BMC Microbiol. 2009 Dec 15;9:259
pubmed: 20003481
BMC Microbiol. 2017 Jul 12;17(1):157
pubmed: 28701186
Nat Rev Microbiol. 2018 Jul;16(7):397-409
pubmed: 29720707
J Periodontol. 2021 Jan;92(1):21-44
pubmed: 32710810
Acta Biomater. 2016 Apr 01;34:133-142
pubmed: 26876875
Clin Oral Implants Res. 2007 Aug;18(4):501-8
pubmed: 17501978
J Clin Periodontol. 1994 Mar;21(3):189-93
pubmed: 8157772
Sci Rep. 2015 Jun 16;5:10948
pubmed: 26077225
J Periodontol. 2018 Jun;89 Suppl 1:S159-S172
pubmed: 29926952
J Clin Periodontol. 2012 Apr;39(4):400-7
pubmed: 22324415