Transport Across Cell Membranes is Modulated by Lipid Order.
Laurdan
generalized polarization
lipid membrane state
membrane permeability
order disorder phase transitions
wound healing
Journal
Advanced biology
ISSN: 2701-0198
Titre abrégé: Adv Biol (Weinh)
Pays: Germany
ID NLM: 101775319
Informations de publication
Date de publication:
06 2023
06 2023
Historique:
revised:
13
12
2022
received:
20
10
2022
medline:
26
6
2023
pubmed:
19
1
2023
entrez:
18
1
2023
Statut:
ppublish
Résumé
This study measures the uptake of various dyes into HeLa cells and determines simultaneously the degree of membrane lipid chain order on a single cell level by spectral analysis of the membrane-embedded dye Laurdan. First, this study finds that the mean generalized polarization (GP) value of single cells varies within a population in a range that is equivalent to a temperature variation of 9 K. This study exploits this natural variety of membrane order to examine the uptake as a function of GP at constant temperature. It is shown that transport across the cell membrane correlates with the membrane phase state. Specifically, higher membrane transport with increasing lipid chain order is observed. As a result, hypothermal-adapted cells with reduced lipid membrane order show less transport. Environmental factors influence transport as well. While increasing temperature reduces lipid order, it is found that locally high cell densities increase lipid order and in turn lead to increased dye uptake. To demonstrate the physiological relevance, membrane state and transport during an in vitro wound healing process are analyzed. While the uptake within a confluent cell layer is high, it decreases toward the center where the membrane lipid chain order is lowest.
Identifiants
pubmed: 36651118
doi: 10.1002/adbi.202200282
doi:
Substances chimiques
Fluorescent Dyes
0
Membrane Lipids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2200282Informations de copyright
© 2023 The Authors. Advanced Biology published by Wiley-VCH GmbH.
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