G protein-coupled receptor kinase-2 (GRK-2) controls exploration through neuropeptide signaling in Caenorhabditis elegans.
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
01 2023
01 2023
Historique:
received:
02
08
2022
accepted:
12
01
2023
revised:
30
01
2023
pubmed:
19
1
2023
medline:
2
2
2023
entrez:
18
1
2023
Statut:
epublish
Résumé
Animals alter their behavior in manners that depend on environmental conditions as well as their developmental and metabolic states. For example, C. elegans is quiescent during larval molts or during conditions of satiety. By contrast, worms enter an exploration state when removed from food. Sensory perception influences movement quiescence (defined as a lack of body movement), as well as the expression of additional locomotor states in C. elegans that are associated with increased or reduced locomotion activity, such as roaming (exploration behavior) and dwelling (local search). Here we find that movement quiescence is enhanced, and exploration behavior is reduced in G protein-coupled receptor kinase grk-2 mutant animals. grk-2 was previously shown to act in chemosensation, locomotion, and egg-laying behaviors. Using neuron-specific rescuing experiments, we show that GRK-2 acts in multiple ciliated chemosensory neurons to control exploration behavior. grk-2 acts in opposite ways from the cGMP-dependent protein kinase gene egl-4 to control movement quiescence and exploration behavior. Analysis of mutants with defects in ciliated sensory neurons indicates that grk-2 and the cilium-structure mutants act in the same pathway to control exploration behavior. We find that GRK-2 controls exploration behavior in an opposite manner from the neuropeptide receptor NPR-1 and the neuropeptides FLP-1 and FLP-18. Finally, we show that secretion of the FLP-1 neuropeptide is negatively regulated by GRK-2 and that overexpression of FLP-1 reduces exploration behavior. These results define neurons and molecular pathways that modulate movement quiescence and exploration behavior.
Identifiants
pubmed: 36652499
doi: 10.1371/journal.pgen.1010613
pii: PGENETICS-D-22-00899
pmc: PMC9886303
doi:
Substances chimiques
Caenorhabditis elegans Proteins
0
Neuropeptides
0
Receptors, G-Protein-Coupled
0
EGL-4 protein, C elegans
EC 2.7.11.12
Cyclic GMP-Dependent Protein Kinases
EC 2.7.11.12
Flp-1 protein, C elegans
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1010613Subventions
Organisme : NIEHS NIH HHS
ID : T32 ES019851
Pays : United States
Organisme : NIEHS NIH HHS
ID : P30 ES013508
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS109476
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS122779
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS107969
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM121481
Pays : United States
Informations de copyright
Copyright: © 2023 Davis et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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