Exploring the interaction of myricetin with human alpha-2-macroglobulin: biophysical and in-silico analysis.
Alpha-2-macroglobulin
CD
FRET
Flavonoids
Molecular docking
Myricetin
Protease inhibitor
Spectroscopy
α2M
Journal
Journal of biological physics
ISSN: 1573-0689
Titre abrégé: J Biol Phys
Pays: Netherlands
ID NLM: 0417731
Informations de publication
Date de publication:
03 2023
03 2023
Historique:
received:
04
10
2022
accepted:
26
12
2022
pubmed:
21
1
2023
medline:
3
3
2023
entrez:
20
1
2023
Statut:
ppublish
Résumé
Myricetin (MYR) is a bioactive secondary metabolite found in plants that is recognized for its nutraceutical value and is an essential constituent of various foods and beverages. It is reported to exhibit a plethora of activities, including antioxidant, antimicrobial, antidiabetic, anticancer, and anti-inflammatory. Alpha-2-macroglobulin (α2M) is a major plasma anti-proteinase that can inhibit proteinases of both human and non-human origin, regardless of their specificity and catalytic mechanism. Here, we explored the interaction of MYR-α2M using various biochemical and biophysical techniques. It was found that the interaction of MYR brings subtle change in its anti-proteolytic potential and thereby alters its structure and function, as can be seen from absorbance and fluorescence spectroscopy. UV spectroscopy of α2M in presence of MYR indicated the occurrence of hyperchromism, suggesting complex formation. Fluorescence spectroscopy reveals that MYR reduces the fluorescence intensity of native α2M with a shift in the wavelength maxima. At 318.15 K, MYR binds to α2M with a binding constant of 2.4 × 10
Identifiants
pubmed: 36662317
doi: 10.1007/s10867-022-09621-z
pii: 10.1007/s10867-022-09621-z
pmc: PMC9867608
doi:
Substances chimiques
myricetin
76XC01FTOJ
Pregnancy-Associated alpha 2-Macroglobulins
0
Flavonoids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
29-48Subventions
Organisme : King Saud University, Riyadh, Saudi Arabia
ID : RSP-2021/393
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature B.V.
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