Human ELISA Detects anti-SARS-CoV-2 Antibodies in Cats: Seroprevalence and Risk Factors for Virus Spread in Domestic and Stray Cats in Bulgaria.
ELISA
SARS-CoV-2
antibodies
cats
humans
Journal
Veterinary sciences
ISSN: 2306-7381
Titre abrégé: Vet Sci
Pays: Switzerland
ID NLM: 101680127
Informations de publication
Date de publication:
06 Jan 2023
06 Jan 2023
Historique:
received:
05
12
2022
revised:
03
01
2023
accepted:
04
01
2023
entrez:
20
1
2023
pubmed:
21
1
2023
medline:
21
1
2023
Statut:
epublish
Résumé
The aim of this study was to verify whether the human DR-ELISA for the detection of anti-SARS-CoV-2 antibodies can be applied in cats, and to assess the risk factors that determine the spread of the virus among the cat population in Bulgaria. The study included 92 serum samples collected from 68 domestic and 24 stray cats aged from 3 months to 20 years of age in the period of January-June 2021. The samples originated from three regions in Bulgaria and from three places of inhabitance. DR-ELISA based on peroxidase-labeled SARS-CoV-2 N protein was employed to detect IgA, IgG and IgM antibodies in the samples. Subsequently, the results were compared with a commercially available multi-species ELISA kit. There was high seroprevalence (83.33%) in stray cats and 41.18% in domestic cats, confirmed by the human and veterinary ELISA kit. The positive cases in the regional cities were 42.86%, in small towns 50% and in villages 78.26%. Cats under 7 years had a five times higher risk than those over 7 years (
Identifiants
pubmed: 36669043
pii: vetsci10010042
doi: 10.3390/vetsci10010042
pmc: PMC9861929
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Medical University of Sofia
ID : D-80/04.06.2021
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