SPIM-Flow: An Integrated Light Sheet and Microfluidics Platform for Hydrodynamic Studies of
Hydra
hydrodynamics
light sheet
microfluidics
Journal
Biology
ISSN: 2079-7737
Titre abrégé: Biology (Basel)
Pays: Switzerland
ID NLM: 101587988
Informations de publication
Date de publication:
11 Jan 2023
11 Jan 2023
Historique:
received:
06
12
2022
revised:
08
01
2023
accepted:
09
01
2023
entrez:
21
1
2023
pubmed:
22
1
2023
medline:
22
1
2023
Statut:
epublish
Résumé
Selective plane illumination microscopy (SPIM), or light sheet microscopy, is a powerful imaging approach. However, access to and interfacing microscopes with microfluidics have remained challenging. Complex interfacing with microfluidics has limited the SPIM's utility for studying the hydrodynamics of freely moving multicellular organisms. We developed SPIM-Flow, an inexpensive light sheet platform that enables easy integration with microfluidics. We used SPIM-Flow to investigate the hydrodynamics of a freely moving
Identifiants
pubmed: 36671808
pii: biology12010116
doi: 10.3390/biology12010116
pmc: PMC9856110
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : CSUPERB
ID : new investigator grant
Organisme : NIH HHS
ID : R21GM135493
Pays : United States
Organisme : National Science Foundation
ID : 2021317020
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