A Novel Window into Angiogenesis-Intravital Microscopy in the AV-Loop-Model.
GelMA
arteriovenous loop
intravital microscopy
tissue engineering
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
09 01 2023
09 01 2023
Historique:
received:
11
12
2022
revised:
04
01
2023
accepted:
06
01
2023
entrez:
21
1
2023
pubmed:
22
1
2023
medline:
25
1
2023
Statut:
epublish
Résumé
Due to the limitations of current in vivo experimental designs, our comprehensive knowledge of vascular development and its implications for the development of large-scale engineered tissue constructs is very limited. Therefore, the purpose of this study was to develop unique in vivo imaging chambers that allow the live visualization of cellular processes in the arteriovenous (AV) loop model in rats. We have developed two different types of chambers. Chamber A is installed in the skin using the purse sting fixing method, while chamber B is installed subcutaneously under the skin. Both chambers are filled with modified gelatin hydrogel as a matrix. Intravital microscopy (IVM) was performed after the injection of fluorescein isothiocyanate (FITC)-labeled dextran and rhodamine 6G dye. The AV loop was functional for two weeks in chamber A and allowed visualization of the leukocyte trafficking. In chamber B, microvascular development in the AV loop could be examined for 21 days. Quantification of the microvascular outgrowth was performed using Fiji-ImageJ. Overall, by combining these two IVM chambers, we can comprehensively understand vascular development in the AV loop tissue engineering model¯.
Identifiants
pubmed: 36672196
pii: cells12020261
doi: 10.3390/cells12020261
pmc: PMC9857023
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Références
PLoS One. 2020 Jan 13;15(1):e0227563
pubmed: 31929570
Acta Biomater. 2018 Dec;82:24-33
pubmed: 30296618
Cells. 2022 Apr 24;11(9):
pubmed: 35563742
J Pers Med. 2022 Feb 08;12(2):
pubmed: 35207725
Medicina (Kaunas). 2020 Nov 23;56(11):
pubmed: 33238390
Adv Mater. 2022 Mar;34(12):e2105883
pubmed: 34773667
Ann Plast Surg. 2008 Jan;60(1):41-4
pubmed: 18281794
Tissue Eng Part C Methods. 2021 Jun;27(6):357-365
pubmed: 33906430
Tissue Eng Part A. 2021 Jan;27(1-2):129-141
pubmed: 32524901
J Pers Med. 2022 Mar 11;12(3):
pubmed: 35330443
Biomed Mater. 2021 Sep 06;16(6):
pubmed: 34406979
Tissue Eng Part A. 2015 May;21(9-10):1680-94
pubmed: 25760576
Handchir Mikrochir Plast Chir. 2006 Aug;38(4):217-23
pubmed: 16991041
Sci Rep. 2019 Jul 19;9(1):10478
pubmed: 31324837
Front Bioeng Biotechnol. 2021 Feb 17;9:627462
pubmed: 33681162
FASEB J. 2007 Feb;21(2):511-22
pubmed: 17172640
J Tissue Eng Regen Med. 2017 Feb;11(2):542-552
pubmed: 26251084
Tissue Eng Part A. 2015 Jan;21(1-2):96-105
pubmed: 25135080
Mater Sci Eng C Mater Biol Appl. 2012 Aug 1;32(6):1536-41
pubmed: 24364957
Nat Rev Immunol. 2006 Jul;6(7):497-507
pubmed: 16799470
NPJ Regen Med. 2021 Aug 19;6(1):49
pubmed: 34413320
Eur Surg Res. 2018;59(3-4):286-299
pubmed: 30244238
Microsurgery. 2018 Sep;38(6):706-717
pubmed: 28738446
J Biomed Mater Res A. 2022 Sep;110(9):1551-1563
pubmed: 35484827
J Pers Med. 2022 Mar 16;12(3):
pubmed: 35330481
Adv Drug Deliv Rev. 2021 Aug;175:113795
pubmed: 33989702
Plast Reconstr Surg. 2012 Jan;129(1):55e-65e
pubmed: 22186585
Nat Rev Cancer. 2023 Jan;23(1):25-42
pubmed: 36385560
J Clin Med. 2022 Aug 31;11(17):
pubmed: 36079063
Plast Reconstr Surg. 2003 Nov;112(6):1636-44
pubmed: 14578795
Nat Protoc. 2021 Apr;16(4):1907-1935
pubmed: 33627843
Tissue Eng. 2007 Jul;13(7):1549-60
pubmed: 17518756
Nat Photonics. 2013 Mar 1;7(3):
pubmed: 24353743
Tissue Eng Part A. 2019 Nov;25(21-22):1504-1513
pubmed: 30848159
Adv Drug Deliv Rev. 2020;158:116-139
pubmed: 32987094
Bioengineering (Basel). 2018 Jul 18;5(3):
pubmed: 30022000
J Vis Exp. 2016 Nov 2;(117):
pubmed: 27842348
NPJ Regen Med. 2022 Feb 23;7(1):17
pubmed: 35197483
Cancer Rep (Hoboken). 2020 Feb;3(1):e1192
pubmed: 32368722
Biochemistry (Mosc). 2019 Jan;84(Suppl 1):S69-S88
pubmed: 31213196
Front Bioeng Biotechnol. 2022 Feb 04;10:820835
pubmed: 35186904
Nat Nanotechnol. 2022 Jun;17(6):653-660
pubmed: 35606441
Bioengineering (Basel). 2022 Jun 27;9(7):
pubmed: 35877331
NPJ Regen Med. 2017;2:
pubmed: 29057097