siRNA-Mediated MELK Knockdown Induces Accelerated Wound Healing with Increased Collagen Deposition.
MELK
fibroblasts
keratinocytes
mice
proliferation
rodents
siRNA
wound healing
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
10 Jan 2023
10 Jan 2023
Historique:
received:
09
12
2022
revised:
22
12
2022
accepted:
06
01
2023
entrez:
21
1
2023
pubmed:
22
1
2023
medline:
25
1
2023
Statut:
epublish
Résumé
Skin wounds remain a significant problem for the healthcare system, affecting the clinical outcome, patients' quality of life, and financial costs. Reduced wound healing times would improve clinical, economic, and social aspects for both patients and the healthcare system. Skin wound healing has been studied for years, but effective therapy that leads to accelerated wound healing remains to be discovered. This study aimed to evaluate the potential of MELK silencing to accelerate wound healing. A vectorless, transient knockdown of the MELK gene using siRNA was performed in a murine skin wound model. The wound size, total collagen, type 3 collagen, vessel size, vessel number, cell proliferation, cell apoptosis, number of mast cells, and immune infiltration by CD45, CD11b, CD45, and CD8a cells were evaluated. We observed that treatment with MELK siRNA leads to significantly faster wound closing associated with increased collagen deposition.
Identifiants
pubmed: 36674843
pii: ijms24021326
doi: 10.3390/ijms24021326
pmc: PMC9861445
pii:
doi:
Substances chimiques
RNA, Small Interfering
0
Collagen
9007-34-5
MELK protein, human
EC 2.7.1.-
Protein Serine-Threonine Kinases
EC 2.7.11.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Polish Ministry of National Defense
ID : KOSCIUSZKO 1 no.571/2016/DA
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