Inhalable Polymeric Nanoparticles for Pulmonary Delivery of Antimicrobial Peptide SET-M33: Antibacterial Activity and Toxicity In Vitro and In Vivo.

Pseudomonas aeruginosa aerosolization antimicrobial peptides antimicrobial resistance inhalable formulations lung delivery nanoparticle properties nanoparticle/mucus interactions polymeric nanoparticles

Journal

Pharmaceutics
ISSN: 1999-4923
Titre abrégé: Pharmaceutics
Pays: Switzerland
ID NLM: 101534003

Informations de publication

Date de publication:
20 Dec 2022
Historique:
received: 11 10 2022
revised: 12 12 2022
accepted: 14 12 2022
entrez: 21 1 2023
pubmed: 22 1 2023
medline: 22 1 2023
Statut: epublish

Résumé

Development of inhalable formulations for delivering peptides to the conductive airways and shielding their interactions with airway barriers, thus enhancing peptide/bacteria interactions, is an important part of peptide-based drug development for lung applications. Here, we report the construction of a biocompatible nanosystem where the antimicrobial peptide SET-M33 is encapsulated within polymeric nanoparticles of poly(lactide-co-glycolide) (PLGA) conjugated with polyethylene glycol (PEG). This system was conceived for better delivery of the peptide to the lungs by aerosol. The encapsulated peptide showed prolonged antibacterial activity, due to its controlled release, and much lower toxicity than the free molecule. The peptide-based nanosystem killed

Identifiants

pubmed: 36678633
pii: pharmaceutics15010003
doi: 10.3390/pharmaceutics15010003
pmc: PMC9863998
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Fondazione Italiana Fibrosi Cistica
ID : FFC#17/2016
Organisme : Regione Toscana
ID : APICECUPI69C20000320002

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Auteurs

Laura Cresti (L)

Laboratory of Clinical Pathology, Santa Maria alle Scotte University Hospital, 53100 Siena, Italy.
SetLance srl, 53100 Siena, Italy.
Department of Medical Biotechnology, University of Siena, 53100 Siena, Italy.

Gemma Conte (G)

Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", 81100 Caserta, Italy.

Giovanni Cappello (G)

SetLance srl, 53100 Siena, Italy.
Department of Medical Biotechnology, University of Siena, 53100 Siena, Italy.

Jlenia Brunetti (J)

Department of Medical Biotechnology, University of Siena, 53100 Siena, Italy.

Chiara Falciani (C)

Department of Medical Biotechnology, University of Siena, 53100 Siena, Italy.

Luisa Bracci (L)

Laboratory of Clinical Pathology, Santa Maria alle Scotte University Hospital, 53100 Siena, Italy.
Department of Medical Biotechnology, University of Siena, 53100 Siena, Italy.

Fabiana Quaglia (F)

Department of Pharmacy, University of Naples Federico II, 80131 Napoli, Italy.

Francesca Ungaro (F)

Department of Pharmacy, University of Naples Federico II, 80131 Napoli, Italy.

Ivana d'Angelo (I)

Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", 81100 Caserta, Italy.

Alessandro Pini (A)

Laboratory of Clinical Pathology, Santa Maria alle Scotte University Hospital, 53100 Siena, Italy.
Department of Medical Biotechnology, University of Siena, 53100 Siena, Italy.

Classifications MeSH