Master mitotic kinases regulate viral genome delivery during papillomavirus cell entry.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
23 01 2023
23 01 2023
Historique:
received:
19
10
2021
accepted:
05
01
2023
entrez:
22
1
2023
pubmed:
23
1
2023
medline:
25
1
2023
Statut:
epublish
Résumé
Mitosis induces cellular rearrangements like spindle formation, Golgi fragmentation, and nuclear envelope breakdown. Similar to certain retroviruses, nuclear delivery during entry of human papillomavirus (HPV) genomes is facilitated by mitosis, during which minor capsid protein L2 tethers viral DNA to mitotic chromosomes. However, the mechanism of viral genome delivery and tethering to condensed chromosomes is barely understood. It is unclear, which cellular proteins facilitate this process or how this process is regulated. This work identifies crucial phosphorylations on HPV minor capsid protein L2 occurring at mitosis onset. L2's chromosome binding region (CBR) is sequentially phosphorylated by the master mitotic kinases CDK1 and PLK1. L2 phosphorylation, thus, regulates timely delivery of HPV vDNA to mitotic chromatin during mitosis. In summary, our work demonstrates a crucial role of mitotic kinases for nuclear delivery of viral DNA and provides important insights into the molecular mechanism of pathogen import into the nucleus during mitosis.
Identifiants
pubmed: 36683055
doi: 10.1038/s41467-023-35874-w
pii: 10.1038/s41467-023-35874-w
pmc: PMC9868124
doi:
Substances chimiques
Capsid Proteins
0
DNA, Viral
0
Cell Cycle Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
355Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM136853
Pays : United States
Informations de copyright
© 2023. The Author(s).
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