Solithromycin inhibits IL-13-induced goblet cell hyperplasia and MUC5AC, CLCA1, and ANO1 in human bronchial epithelial cells.


Journal

PeerJ
ISSN: 2167-8359
Titre abrégé: PeerJ
Pays: United States
ID NLM: 101603425

Informations de publication

Date de publication:
2023
Historique:
received: 12 07 2022
accepted: 14 12 2022
entrez: 23 1 2023
pubmed: 24 1 2023
medline: 25 1 2023
Statut: epublish

Résumé

Solithromycin is a novel fluoroketolide antibiotic belonging to the class of macrolide antibiotics. Activation of the interleukin (IL)-13 receptor leads to STAT6 activation and subsequent induction of SAM pointed domain containing ETS transcription factor (SPDEF), chloride channel accessory 1 (CLCA1), and anoctamin-1 (ANO1), all of which are associated with the induction of MUC5AC. We examined the effects of solithromycin on mucin production led by IL-13 signaling. Normal human bronchial epithelial cells were grown at the air-liquid interface with IL-13 with/without solithromycin for 14 days. Histochemical analysis was performed using hematoxylin and eosin staining and MUC5AC immunostaining.

Identifiants

pubmed: 36684665
doi: 10.7717/peerj.14695
pii: 14695
pmc: PMC9854378
doi:

Substances chimiques

ANO1 protein, human 0
Anoctamin-1 0
Chloride Channels 0
CLCA1 protein, human 0
Interleukin-13 0
Macrolides 0
MUC5AC protein, human 0
Mucin 5AC 0
Neoplasm Proteins 0
RNA, Messenger 0
solithromycin 9U1ETH79CK

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e14695

Informations de copyright

©2023 Kimura et al.

Déclaration de conflit d'intérêts

The authors declare there are no competing interests.

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Auteurs

Yasuhiro Kimura (Y)

Department of Pulmonology, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.

Masahiro Shinoda (M)

Department of Respiratory Medicine, Tokyo Shinagawa Hospital, Shinagawa, Tokyo, Japan.

Masaharu Shinkai (M)

Department of Respiratory Medicine, Tokyo Shinagawa Hospital, Shinagawa, Tokyo, Japan.

Takeshi Kaneko (T)

Department of Pulmonology, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.

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