Identification of extremely GC-rich micro RNAs for RT-qPCR data normalization in human plasma.

cognitive stress-coping human plasma miRNAs miRNA expression microarray miRNA reference genes qPCR normalization small-RNA sequencing stem-loop reverse-transcription quantitative PCR

Journal

Frontiers in genetics
ISSN: 1664-8021
Titre abrégé: Front Genet
Pays: Switzerland
ID NLM: 101560621

Informations de publication

Date de publication:
2022
Historique:
received: 30 09 2022
accepted: 02 12 2022
entrez: 23 1 2023
pubmed: 24 1 2023
medline: 24 1 2023
Statut: epublish

Résumé

We aimed at extending the repertoire of high-quality miRNA normalizers for reverse transcription-quantitative PCR (RT-qPCR) of human plasma with special emphasis on the extremely guanine-cytosine-rich portion of the miRNome. For high-throughput selection of stable candidates, microarray technology was preferred over small-RNA sequencing (sRNA-seq) since the latter underrepresented miRNAs with a guanine-cytosine (GC) content of at least 75% (

Identifiants

pubmed: 36685854
doi: 10.3389/fgene.2022.1058668
pii: 1058668
pmc: PMC9846067
doi:

Types de publication

Journal Article

Langues

eng

Pagination

1058668

Commentaires et corrections

Type : ErratumIn

Informations de copyright

Copyright © 2023 Baumann, Athanasiou, Faridani, Schwerdtfeger, Wallner and Steinborn.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Volker Baumann (V)

Genomics Core Facility, VetCore, University of Veterinary Medicine, Vienna, Austria.

Angelos-Theodoros Athanasiou (AT)

Genomics Core Facility, VetCore, University of Veterinary Medicine, Vienna, Austria.

Omid R Faridani (OR)

Garvan Institute of Medical Research, Sydney, NSW, Australia.
Lowy Cancer Research Centre, School of Biomedical Sciences, University of New South Wales, Sydney, NSW, Australia.

Andreas R Schwerdtfeger (AR)

Institute of Psychology, Karl-Franzens-University Graz, Graz, Austria.

Bernard Wallner (B)

Department of Behavioral and Cognitive Biology, University of Vienna, Vienna, Austria.

Ralf Steinborn (R)

Genomics Core Facility, VetCore, University of Veterinary Medicine, Vienna, Austria.
Department of Microbiology, Immunobiology and Genetics, University of Vienna, Vienna, Austria.

Classifications MeSH