Whole-mouse clearing and imaging at the cellular level with vDISCO.

Mice Animals Imaging, Three-Dimensional / methods Microscopy, Fluorescence / methods Brain Solvents / chemistry Neurites Coloring Agents

Journal

Nature protocols
ISSN: 1750-2799
Titre abrégé: Nat Protoc
Pays: England
ID NLM: 101284307

Informations de publication

Date de publication:
04 2023
Historique:
received: 29 09 2021
accepted: 20 10 2022
medline: 13 4 2023
pubmed: 26 1 2023
entrez: 25 1 2023
Statut: ppublish

Résumé

Homeostatic and pathological phenomena often affect multiple organs across the whole organism. Tissue clearing methods, together with recent advances in microscopy, have made holistic examinations of biological samples feasible. Here, we report the detailed protocol for nanobody(V

Identifiants

DOI: 10.1038/s41596-022-00788-2 PMID: 36697871
pubmed: 36697871
doi: 10.1038/s41596-022-00788-2
pii: 10.1038/s41596-022-00788-2
doi:

Substances chimiques

Solvents 0
Coloring Agents 0

Types de publication

Journal Article Review Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1197-1242

Informations de copyright

© 2023. Springer Nature Limited.

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Auteurs

Ruiyao Cai (R)

Institute for Tissue Engineering and Regenerative Medicine (iTERM), Helmholtz Munich, Munich, Germany.
Institute for Stroke and Dementia Research, Klinikum der Universität München, Ludwig Maximilian University of Munich, Munich, Germany.

Zeynep Ilgin Kolabas (ZI)

Institute for Tissue Engineering and Regenerative Medicine (iTERM), Helmholtz Munich, Munich, Germany.
Institute for Stroke and Dementia Research, Klinikum der Universität München, Ludwig Maximilian University of Munich, Munich, Germany.
Graduate School of Systemic Neurosciences (GSN), Munich, Germany.

Chenchen Pan (C)

Institute for Tissue Engineering and Regenerative Medicine (iTERM), Helmholtz Munich, Munich, Germany.
Institute for Stroke and Dementia Research, Klinikum der Universität München, Ludwig Maximilian University of Munich, Munich, Germany.

Hongcheng Mai (H)

Institute for Tissue Engineering and Regenerative Medicine (iTERM), Helmholtz Munich, Munich, Germany.
Institute for Stroke and Dementia Research, Klinikum der Universität München, Ludwig Maximilian University of Munich, Munich, Germany.
ORCID: 0000-0001-6615-0157

Shan Zhao (S)

Institute for Tissue Engineering and Regenerative Medicine (iTERM), Helmholtz Munich, Munich, Germany.
Institute for Stroke and Dementia Research, Klinikum der Universität München, Ludwig Maximilian University of Munich, Munich, Germany.

Doris Kaltenecker (D)

Institute for Tissue Engineering and Regenerative Medicine (iTERM), Helmholtz Munich, Munich, Germany.
Institute for Stroke and Dementia Research, Klinikum der Universität München, Ludwig Maximilian University of Munich, Munich, Germany.
Institute for Diabetes and Cancer, Helmholtz Munich, Munich, Germany.

Fabian F Voigt (FF)

Brain Research Institute, University of Zurich, Zurich, Switzerland.
Neuroscience Center Zurich, University of Zurich, Zurich, Switzerland.
ORCID: 0000-0002-7108-2176

Muge Molbay (M)

Institute for Tissue Engineering and Regenerative Medicine (iTERM), Helmholtz Munich, Munich, Germany.
Institute for Stroke and Dementia Research, Klinikum der Universität München, Ludwig Maximilian University of Munich, Munich, Germany.

Tzu-Lun Ohn (TL)

Institute for Tissue Engineering and Regenerative Medicine (iTERM), Helmholtz Munich, Munich, Germany.
Institute for Stroke and Dementia Research, Klinikum der Universität München, Ludwig Maximilian University of Munich, Munich, Germany.

Cécile Vincke (C)

Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium.
Myeloid Cell Immunology Lab, VIB Center for Inflammation Research, Brussels, Belgium.

Mihail I Todorov (MI)

Institute for Tissue Engineering and Regenerative Medicine (iTERM), Helmholtz Munich, Munich, Germany.
Institute for Stroke and Dementia Research, Klinikum der Universität München, Ludwig Maximilian University of Munich, Munich, Germany.

Fritjof Helmchen (F)

Brain Research Institute, University of Zurich, Zurich, Switzerland.
Neuroscience Center Zurich, University of Zurich, Zurich, Switzerland.
ORCID: 0000-0002-8867-9569

Jo A Van Ginderachter (JA)

Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium.
Myeloid Cell Immunology Lab, VIB Center for Inflammation Research, Brussels, Belgium.

Ali Ertürk (A)

Institute for Tissue Engineering and Regenerative Medicine (iTERM), Helmholtz Munich, Munich, Germany. erturk@helmholtz-muenchen.de.
Institute for Stroke and Dementia Research, Klinikum der Universität München, Ludwig Maximilian University of Munich, Munich, Germany. erturk@helmholtz-muenchen.de.
Munich Cluster for Systems Neurology (SyNergy), Munich, Germany. erturk@helmholtz-muenchen.de.
ORCID: 0000-0001-5163-5100

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Whole-mouse clearing and imaging at the...
questionsmedicales.fr › Eucaryotes › Animaux › Whole-mouse clearing and imaging at the...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Traitement d'image par ordinateur › Imagerie tridimensionnelle › Whole-mouse clearing and imaging at the...
questionsmedicales.fr › Techniques d'investigation › Microscopie de fluorescence › Whole-mouse clearing and imaging at the...
questionsmedicales.fr › Système nerveux › Système nerveux central › Encéphale › Whole-mouse clearing and imaging at the...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Solvants › Whole-mouse clearing and imaging at the...
questionsmedicales.fr › Cellules › Neurones › Neurites › Whole-mouse clearing and imaging at the...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Agents colorants › Whole-mouse clearing and imaging at the...