Plasmon-Enhanced Single Extracellular Vesicle Analysis for Cholangiocarcinoma Diagnosis.
biomarkers
cholangiocarcinoma
diagnostics
extracellular vesicles
plasmonics
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
03 2023
03 2023
Historique:
revised:
01
01
2023
received:
07
09
2022
pubmed:
27
1
2023
medline:
21
3
2023
entrez:
26
1
2023
Statut:
ppublish
Résumé
Cholangiocarcinoma (CCA) is a fatal disease often detected late in unresectable stages. Currently, there are no effective diagnostic methods or biomarkers to detect CCA early with high confidence. Analysis of tumor-derived extracellular vesicles (tEVs) harvested from liquid biopsies can provide a new opportunity to achieve this goal. Here, an advanced nanoplasmonic sensing technology is reported, termed FLEX (fluorescence-amplified extracellular vesicle sensing technology), for sensitive and robust single EV analysis. In the FLEX assay, EVs are captured on a plasmonic gold nanowell surface and immunolabeled for cancer-associated biomarkers to identify tEVs. The underlying plasmonic gold nanowell structures then amplify EVs' fluorescence signals, an effective amplification process at the single EV level. The FLEX EV analysis revealed a wide heterogeneity of tEVs and their marker levels. FLEX also detected small tEVs not detected by conventional EV fluorescence imaging due to weak signals. Tumor markers (MUC1, EGFR, and EPCAM) are identified in CCA, and this marker combination is applied to detect tEVs in clinical bile samples. The FLEX assay detected CCA with an area under the curve of 0.93, significantly better than current clinical markers. The sensitive and accurate nanoplasmonic EV sensing technology can aid in early CCA diagnosis.
Identifiants
pubmed: 36698298
doi: 10.1002/advs.202205148
pmc: PMC10015870
doi:
Substances chimiques
Biomarkers, Tumor
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2205148Subventions
Organisme : Severance Hospital Research Fund for Clinical Excellence
ID : C-2019-0036
Organisme : NIGMS NIH HHS
ID : R01GM138778
Pays : United States
Organisme : Ministry of Trade, Industry, and Energy, Republic of Korea
ID : 20008829
Organisme : Severance Hospital Research Fund for Clinical Excellence
ID : C-2018-0008
Organisme : NIGMS NIH HHS
ID : R01 GM138778
Pays : United States
Organisme : National Research Foundation of Korea
ID : NRF-2021R1A6A3A14039686
Organisme : NCI NIH HHS
ID : R21CA217662
Pays : United States
Organisme : NCI NIH HHS
ID : R21 CA217662
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01GM138778
Pays : United States
Organisme : NCI NIH HHS
ID : R21CA217662
Pays : United States
Informations de copyright
© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.
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