scMEGA: single-cell multi-omic enhancer-based gene regulatory network inference.


Journal

Bioinformatics advances
ISSN: 2635-0041
Titre abrégé: Bioinform Adv
Pays: England
ID NLM: 9918282081306676

Informations de publication

Date de publication:
2023
Historique:
received: 18 10 2022
revised: 08 12 2022
accepted: 11 01 2023
entrez: 26 1 2023
pubmed: 27 1 2023
medline: 27 1 2023
Statut: epublish

Résumé

The increasing availability of single-cell multi-omics data allows to quantitatively characterize gene regulation. We here describe scMEGA (Single-cell Multiomic Enhancer-based Gene Regulatory Network Inference) that enables an end-to-end analysis of multi-omics data for gene regulatory network inference including modalities integration, trajectory analysis, enhancer-to-promoter association, network analysis and visualization. This enables to study the complex gene regulation mechanisms for dynamic biological processes, such as cellular differentiation and disease-driven cellular remodeling. We provide a case study on gene regulatory networks controlling myofibroblast activation in human myocardial infarction. scMEGA is implemented in R, released under the MIT license and available from https://github.com/CostaLab/scMEGA. Tutorials are available from https://costalab.github.io/scMEGA. Supplementary data are available at

Identifiants

pubmed: 36698768
doi: 10.1093/bioadv/vbad003
pii: vbad003
pmc: PMC9853317
doi:

Types de publication

Journal Article

Langues

eng

Pagination

vbad003

Informations de copyright

© The Author(s) 2023. Published by Oxford University Press.

Références

Nature. 2021 Jan;589(7841):281-286
pubmed: 33176333
Nat Methods. 2020 Jan;17(1):11-14
pubmed: 31907462
Cell. 2020 Nov 12;183(4):1103-1116.e20
pubmed: 33098772
Nature. 2022 Jan;601(7894):630-636
pubmed: 34987221
Nat Commun. 2021 Nov 4;12(1):6386
pubmed: 34737275
Nat Methods. 2019 Dec;16(12):1289-1296
pubmed: 31740819
Nature. 2022 Oct 5;:
pubmed: 36198796
Nat Methods. 2017 Oct;14(10):975-978
pubmed: 28825706
Nature. 2022 Aug;608(7924):766-777
pubmed: 35948637
Bioinformatics. 2022 Jun 24;38(Suppl 1):i282-i289
pubmed: 35758807
Cell. 2019 Jun 13;177(7):1888-1902.e21
pubmed: 31178118
Sci Rep. 2021 Mar 1;11(1):4919
pubmed: 33649386
Nat Genet. 2021 Mar;53(3):403-411
pubmed: 33633365
JCI Insight. 2017 Sep 7;2(17):
pubmed: 28878117
Nat Commun. 2011 Jul 19;2:394
pubmed: 21772265
Cell Genom. 2022 Sep 14;2(9):
pubmed: 36204155

Auteurs

Zhijian Li (Z)

Institute for Computational Genomics, Joint Research Center for Computational Biomedicine, RWTH Aachen University Medical School, Aachen 52062, Germany.

James S Nagai (JS)

Institute for Computational Genomics, Joint Research Center for Computational Biomedicine, RWTH Aachen University Medical School, Aachen 52062, Germany.

Christoph Kuppe (C)

Institute of Experimental Medicine and Systems Biology, RWTH Aachen University, Aachen 52062, Germany.
Division of Nephrology and Clinical Immunology, RWTH Aachen University, Aachen 52062, Germany.

Rafael Kramann (R)

Institute of Experimental Medicine and Systems Biology, RWTH Aachen University, Aachen 52062, Germany.
Division of Nephrology and Clinical Immunology, RWTH Aachen University, Aachen 52062, Germany.
Department of Internal Medicine, Nephrology and Transplantation, Erasmus Medical Center, Rotterdam 3042, The Netherlands.

Ivan G Costa (IG)

Institute for Computational Genomics, Joint Research Center for Computational Biomedicine, RWTH Aachen University Medical School, Aachen 52062, Germany.

Classifications MeSH