Validation of a fully automated chemiluminescent immunoassay for cattle serum and plasma progesterone measurement.
cattle
immunoassay
plasma
progesterone
serum
validation
Journal
Frontiers in veterinary science
ISSN: 2297-1769
Titre abrégé: Front Vet Sci
Pays: Switzerland
ID NLM: 101666658
Informations de publication
Date de publication:
2022
2022
Historique:
received:
07
10
2022
accepted:
20
12
2022
entrez:
26
1
2023
pubmed:
27
1
2023
medline:
27
1
2023
Statut:
epublish
Résumé
Monitoring circulating progesterone concentration ([P4]) is an important component of basic and applied reproduction research and clinical settings. IMMULITE Our objective was therefore to characterize the analytical performance of the IMMULITE The IPI validation protocols included characterization of the method linearity, within-run, and between-run precision through calculation of the coefficient of variation (CV). The method accuracy was assessed through the calculation of the spiking-recovery (SR) bias across the reportable range (0.2-40.0 ng/mL). Passing-Bablok regression and Bland-Altman plots were used to determine the interlaboratory bias for two laboratories. Three types of observed total error (TEo) were calculated based on the considered type of bias, TEo The IPI was linearly related to the true value ( We concluded that the automated IPI provides a precise, accurate, reliable, and safe method for measuring [P4] in both serum and plasma of cattle. Consistent with the manufacturer's recommendations, the serum matrix is more accurate than plasma.
Identifiants
pubmed: 36699340
doi: 10.3389/fvets.2022.1064201
pmc: PMC9868466
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1064201Informations de copyright
Copyright © 2023 Megahed, Jones, Bisinotto, Chebel, Galvão, Chan and Bittar.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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