Quantification of phospholipids and glycerides in human milk using ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry.
UPLC-Q-ToF-MS
gangliosides
glycerides
human milk
lipids
phospholipids
quantification
Journal
Frontiers in chemistry
ISSN: 2296-2646
Titre abrégé: Front Chem
Pays: Switzerland
ID NLM: 101627988
Informations de publication
Date de publication:
2022
2022
Historique:
received:
18
11
2022
accepted:
23
12
2022
entrez:
26
1
2023
pubmed:
27
1
2023
medline:
27
1
2023
Statut:
epublish
Résumé
Human milk lipids, which are an important source of energy and affect growth and development of infants, require a comprehensive method for its qualitative and quantitative analysis. This work describes a method for the analysis of phospholipids, glycerides, free fatty acids and gangliosides in human milk by ultra-performance liquid chromatography using a C18 column with quadrupole-time-of-flight mass spectrometry (Q-TOF-MS). The lipids were extracted by liquid-liquid extraction and phospholipids were separated by solid phase extraction (SPE). The chromatographic columns with two different specifications (4.6 mm × 150 mm, and 3 mm × 50 mm) were used to detect phospholipids and glycerides in human milk, respectively. The sphingolipids and glycerides were analyzed in positive ion mode, and the glycerophospholipids and free fatty acids were analyzed in negative ion mode. Both internal and external standards were used for absolute quantification in this experiment. 483 species of lipids, including phospholipids, glycerides, free fatty acids and gangliosides, in human milk were analyzed using UPLC-Q-TOF-MS with high sensitivity and good linearity, with coefficient of correlation above 0.99, the relative standard deviation of accuracy and precision less than 10%. The results in a large number of human milk samples showed that this method was suitable for qualitative and quantitative analysis of lipids in human milk, even for other mammalian milk and infant formulae.
Identifiants
pubmed: 36700070
doi: 10.3389/fchem.2022.1101557
pii: 1101557
pmc: PMC9868747
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1101557Informations de copyright
Copyright © 2023 Liu, Qiao, Liu, Zhao, Liu, Yang, Zhang, Wang, Liu and Chen.
Déclaration de conflit d'intérêts
Authors YL, WQ, YL, JZ, QL, KY, MZ, YW, YL, and LC were employed by the company Beijing Sanyuan Foods Co., Ltd. The authors declare that this study received funding from Beijing Sanyuan Foods Co., Ltd. The funder had the following involvement in the study: Maternal and Infant Nutriomics Cohort Study.
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