PoET: automated approach for measuring pore edge tension in giant unilamellar vesicles.
Journal
Bioinformatics advances
ISSN: 2635-0041
Titre abrégé: Bioinform Adv
Pays: England
ID NLM: 9918282081306676
Informations de publication
Date de publication:
2021
2021
Historique:
received:
13
09
2021
revised:
09
11
2021
accepted:
17
11
2021
entrez:
26
1
2023
pubmed:
23
11
2021
medline:
23
11
2021
Statut:
epublish
Résumé
A reliable characterization of the membrane pore edge tension of single giant unilamellar vesicles (GUVs) requires the measurement of micrometer sized pores in hundreds to thousands of images. When manually performed, this procedure has shown to be extremely time-consuming and to generate inconsistent results among different users and imaging systems. A user-friendly software for such analysis allowing quick processing and generation of reproducible data had not yet been reported. We have developed a software (PoET) for automatic pore edge tension measurements on GUVs. The required image processing steps and the characterization of the pore dynamics are performed automatically within the software and its use allowed for a 30-fold reduction in the analysis time. We demonstrate the applicability of the software by comparing the pore edge tension of GUVs of different membrane compositions and surface charges. The approach was applied to electroporated GUVs but is applicable to other means of pore formation. The complete software is implemented in Python and available for Windows at https://dx.doi.org/10.17617/3.7h. Supplementary data are available at
Identifiants
pubmed: 36700098
doi: 10.1093/bioadv/vbab037
pii: vbab037
pmc: PMC9710609
doi:
Types de publication
Journal Article
Langues
eng
Pagination
vbab037Informations de copyright
© The Author(s) 2021. Published by Oxford University Press.
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