An advanced

advanced in vitro model allergenicity risk assessment allergic sensitization food allergy mucosal immunity ovalbumin

Journal

Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960

Informations de publication

Date de publication:
2022
Historique:
received: 18 10 2022
accepted: 19 12 2022
entrez: 26 1 2023
pubmed: 27 1 2023
medline: 28 1 2023
Statut: epublish

Résumé

The global demand of sustainable food sources leads to introduction of novel foods on the market, which may pose a risk of inducing allergic sensitization. Currently there are no validated This Ovalbumin activates IEC and underlying moDCs, both resulting in downstream IgE isotype-switching. However, only direct exposure of moDCs to ovalbumin drives Th2 polarization and a humoral B cell response allowing for IgE mediated mast cell degranulation, IL13 and IL4 release in this sequential DC-T cell-B cell-mast cell model, indicating also an immunomodulatory role for IEC. This

Sections du résumé

Background
The global demand of sustainable food sources leads to introduction of novel foods on the market, which may pose a risk of inducing allergic sensitization. Currently there are no validated
Methods
This
Results
Ovalbumin activates IEC and underlying moDCs, both resulting in downstream IgE isotype-switching. However, only direct exposure of moDCs to ovalbumin drives Th2 polarization and a humoral B cell response allowing for IgE mediated mast cell degranulation, IL13 and IL4 release in this sequential DC-T cell-B cell-mast cell model, indicating also an immunomodulatory role for IEC.
Conclusion
This

Identifiants

pubmed: 36700233
doi: 10.3389/fimmu.2022.1073034
pmc: PMC9869142
doi:

Substances chimiques

Ovalbumin 9006-59-1
Allergens 0
Immunoglobulin E 37341-29-0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1073034

Informations de copyright

Copyright © 2023 Zuurveld, Díaz, Redegeld, Folkerts, Garssen, van’t Land and Willemsen.

Déclaration de conflit d'intérêts

Authors JG and BL are partly employed by Danone Nutricia Research B.V. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Marit Zuurveld (M)

Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, Netherlands.

Cristina Bueno Díaz (CB)

Chemical Biology and Drug Discovery Group, Department of Pharmacological Sciences, Utrecht University, Utrecht, Netherlands.

Frank Redegeld (F)

Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, Netherlands.

Gert Folkerts (G)

Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, Netherlands.

Johan Garssen (J)

Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, Netherlands.
Immunology Platform, Danone Nutricia Research B.V., Utrecht, Netherlands.

Belinda Van't Land (B)

Immunology Platform, Danone Nutricia Research B.V., Utrecht, Netherlands.
Center for Translational Immunology, University Medical Center Utrecht, Utrecht, Netherlands.

Linette E M Willemsen (LEM)

Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, Netherlands.

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Classifications MeSH