Circulating Extracellular Vesicle-Propagated microRNA Signature as a Vascular Calcification Factor in Chronic Kidney Disease.

Rats Mice Animals MicroRNAs / genetics Vascular Endothelial Growth Factor A / genetics Muscle, Smooth, Vascular / metabolism Vascular Calcification / metabolism Renal Insufficiency, Chronic / metabolism Extracellular Vesicles / metabolism Phosphorus / metabolism Myocytes, Smooth Muscle / metabolism
anticoagulant atherosclerosis calcium phosphate cardiovascular disease creatinine glomerular filtration rate

Journal

Circulation research
ISSN: 1524-4571
Titre abrégé: Circ Res
Pays: United States
ID NLM: 0047103

Informations de publication

Date de publication:
17 02 2023
Historique:
pubmed: 27 1 2023
medline: 22 2 2023
entrez: 26 1 2023
Statut: ppublish

Résumé

Chronic kidney disease (CKD) accelerates vascular calcification via phenotypic switching of vascular smooth muscle cells (VSMCs). We investigated the roles of circulating small extracellular vesicles (sEVs) between the kidneys and VSMCs and uncovered relevant sEV-propagated microRNAs (miRNAs) and their biological signaling pathways. We established CKD models in rats and mice by adenine-induced tubulointerstitial fibrosis. Cultures of A10 embryonic rat VSMCs showed increased calcification and transcription of osterix ( The miRNA transcriptomic signature of circulating sEVs uncovered their pathologic role, devoid of the calcification-protective miRNAs that target VEGFA signaling in CKD-driven vascular calcification. These sEV-propagated miRNAs are potential biomarkers and therapeutic targets for vascular calcification.

Sections du résumé

BACKGROUND
Chronic kidney disease (CKD) accelerates vascular calcification via phenotypic switching of vascular smooth muscle cells (VSMCs). We investigated the roles of circulating small extracellular vesicles (sEVs) between the kidneys and VSMCs and uncovered relevant sEV-propagated microRNAs (miRNAs) and their biological signaling pathways.
METHODS AND RESULTS
We established CKD models in rats and mice by adenine-induced tubulointerstitial fibrosis. Cultures of A10 embryonic rat VSMCs showed increased calcification and transcription of osterix (
CONCLUSIONS
The miRNA transcriptomic signature of circulating sEVs uncovered their pathologic role, devoid of the calcification-protective miRNAs that target VEGFA signaling in CKD-driven vascular calcification. These sEV-propagated miRNAs are potential biomarkers and therapeutic targets for vascular calcification.

Identifiants

DOI: 10.1161/CIRCRESAHA.122.321939 PMID: 36700539
pubmed: 36700539
doi: 10.1161/CIRCRESAHA.122.321939
doi:

Substances chimiques

MicroRNAs 0
GW 4869 0
Vascular Endothelial Growth Factor A 0
Phosphorus 27YLU75U4W

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

415-431

Auteurs

Takaaki Koide (T)

Department of Nephrology (T.K., S.M., R.K., H.M., M.C., S.S., T.F., F.A., T.M., K.S., S.I., S.N., E.S., T.R., S.U.), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.
ORCID: 0000-0001-7332-6069

Shintaro Mandai (S)

Department of Nephrology (T.K., S.M., R.K., H.M., M.C., S.S., T.F., F.A., T.M., K.S., S.I., S.N., E.S., T.R., S.U.), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.
ORCID: 0000-0001-6709-306X

Reo Kitaoka (R)

Department of Nephrology (T.K., S.M., R.K., H.M., M.C., S.S., T.F., F.A., T.M., K.S., S.I., S.N., E.S., T.R., S.U.), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.

Hisazumi Matsuki (H)

Department of Nephrology (T.K., S.M., R.K., H.M., M.C., S.S., T.F., F.A., T.M., K.S., S.I., S.N., E.S., T.R., S.U.), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.

Motoko Chiga (M)

Department of Nephrology (T.K., S.M., R.K., H.M., M.C., S.S., T.F., F.A., T.M., K.S., S.I., S.N., E.S., T.R., S.U.), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.

Kouhei Yamamoto (K)

Department of Human Pathology (K. Yamamoto), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.

Kotaro Yoshioka (K)

Department of Neurology and Neurological Science (K. Yoshioka, Y.Y., T.Y.), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.
ORCID: 0000-0002-6490-3263

Yohsuke Yagi (Y)

Department of Neurology and Neurological Science (K. Yoshioka, Y.Y., T.Y.), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.

Soichiro Suzuki (S)

Department of Nephrology (T.K., S.M., R.K., H.M., M.C., S.S., T.F., F.A., T.M., K.S., S.I., S.N., E.S., T.R., S.U.), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.

Tamami Fujiki (T)

Department of Nephrology (T.K., S.M., R.K., H.M., M.C., S.S., T.F., F.A., T.M., K.S., S.I., S.N., E.S., T.R., S.U.), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.

Fumiaki Ando (F)

Department of Nephrology (T.K., S.M., R.K., H.M., M.C., S.S., T.F., F.A., T.M., K.S., S.I., S.N., E.S., T.R., S.U.), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.
ORCID: 0000-0002-8401-1705

Takayasu Mori (T)

Department of Nephrology (T.K., S.M., R.K., H.M., M.C., S.S., T.F., F.A., T.M., K.S., S.I., S.N., E.S., T.R., S.U.), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.
ORCID: 0000-0002-9308-7787

Koichiro Susa (K)

Department of Nephrology (T.K., S.M., R.K., H.M., M.C., S.S., T.F., F.A., T.M., K.S., S.I., S.N., E.S., T.R., S.U.), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.
ORCID: 0000-0002-0100-4527

Soichiro Iimori (S)

Department of Nephrology (T.K., S.M., R.K., H.M., M.C., S.S., T.F., F.A., T.M., K.S., S.I., S.N., E.S., T.R., S.U.), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.

Shotaro Naito (S)

Department of Nephrology (T.K., S.M., R.K., H.M., M.C., S.S., T.F., F.A., T.M., K.S., S.I., S.N., E.S., T.R., S.U.), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.
ORCID: 0000-0001-9774-1638

Eisei Sohara (E)

Department of Nephrology (T.K., S.M., R.K., H.M., M.C., S.S., T.F., F.A., T.M., K.S., S.I., S.N., E.S., T.R., S.U.), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.

Tatemitsu Rai (T)

Department of Nephrology (T.K., S.M., R.K., H.M., M.C., S.S., T.F., F.A., T.M., K.S., S.I., S.N., E.S., T.R., S.U.), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.

Takanori Yokota (T)

Department of Neurology and Neurological Science (K. Yoshioka, Y.Y., T.Y.), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.

Shinichi Uchida (S)

Department of Nephrology (T.K., S.M., R.K., H.M., M.C., S.S., T.F., F.A., T.M., K.S., S.I., S.N., E.S., T.R., S.U.), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Japan.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Circulating Extracellular Vesicle-Propagated...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Circulating Extracellular Vesicle-Propagated...
questionsmedicales.fr › Eucaryotes › Animaux › Circulating Extracellular Vesicle-Propagated...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › microARN › Circulating Extracellular Vesicle-Propagated...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Protéines angiogéniques › Facteurs de croissance endothéliale vasculaire › Facteur de croissance endothéliale vasculaire de type A › Circulating Extracellular Vesicle-Propagated...
questionsmedicales.fr › Tissus › Muscles › Muscles lisses › Muscles lisses vasculaires › Circulating Extracellular Vesicle-Propagated...
questionsmedicales.fr › Maladies métaboliques et nutritionnelles › Maladies métaboliques › Troubles du métabolisme du calcium › Calcinose › Calcification vasculaire › Circulating Extracellular Vesicle-Propagated...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Caractéristiques de la maladie › Maladie chronique › Insuffisance rénale chronique › Circulating Extracellular Vesicle-Propagated...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace extracellulaire › Vésicules extracellulaires › Circulating Extracellular Vesicle-Propagated...
questionsmedicales.fr › Produits chimiques inorganiques › Éléments › Phosphore › Circulating Extracellular Vesicle-Propagated...
questionsmedicales.fr › Cellules › Cellules musculaires › Myocytes du muscle lisse › Circulating Extracellular Vesicle-Propagated...