Click and Detect: Versatile Ampicillin Aptasensor Enabled by Click Chemistry on a Graphene-Alkyne Derivative.
antibiotic detection
aptamers
biosensors
graphene acid
screen printed carbon electrodes
Journal
Small (Weinheim an der Bergstrasse, Germany)
ISSN: 1613-6829
Titre abrégé: Small
Pays: Germany
ID NLM: 101235338
Informations de publication
Date de publication:
26 Jan 2023
26 Jan 2023
Historique:
revised:
20
12
2022
received:
20
11
2022
entrez:
27
1
2023
pubmed:
28
1
2023
medline:
28
1
2023
Statut:
aheadofprint
Résumé
Tackling the current problem of antimicrobial resistance (AMR) requires fast, inexpensive, and effective methods for controlling and detecting antibiotics in diverse samples at the point of interest. Cost-effective, disposable, point-of-care electrochemical biosensors are a particularly attractive option. However, there is a need for conductive and versatile carbon-based materials and inks that enable effective bioconjugation under mild conditions for the development of robust, sensitive, and selective devices. This work describes a simple and fast methodology to construct an aptasensor based on a novel graphene derivative equipped with alkyne groups prepared via fluorographene chemistry. Using click chemistry, an aptamer is immobilized and used as a successful platform for the selective determination of ampicillin in real samples in the presence of interfering molecules. The electrochemical aptasensor displayed a detection limit of 1.36 nM, high selectivity among other antibiotics, the storage stability of 4 weeks, and is effective in real samples. Additionally, structural and docking simulations of the aptamer shed light on the ampicillin binding mechanism. The versatility of this platform opens up wide possibilities for constructing a new class of aptasensor based on disposable screen-printed carbon electrodes usable in point-of-care devices.
Identifiants
pubmed: 36703534
doi: 10.1002/smll.202207216
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2207216Subventions
Organisme : Technology Agency of the Czech Republic, Program TREND
ID : FW01010183
Organisme : ERDF/ESF project "Nano4Future"
ID : CZ.02.1.01/0.0/0.0/16_019/0000754
Organisme : Ministry of Education, Youth and Sports of the Czech Republic
ID : LM2018124
Organisme : European Union's Horizon Europe research and innovation program
ID : 101059266
Organisme : ERC consolidator grant
ID : 683024
Organisme : Czech Science Foundation
ID : 19-27454X
Informations de copyright
© 2023 The Authors. Small published by Wiley-VCH GmbH.
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