Directed differentiation of hPSCs through a simplified lateral plate mesoderm protocol for generation of articular cartilage progenitors.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2023
2023
Historique:
received:
06
06
2022
accepted:
20
12
2022
entrez:
27
1
2023
pubmed:
28
1
2023
medline:
1
2
2023
Statut:
epublish
Résumé
Developmentally, the articular joints are derived from lateral plate (LP) mesoderm. However, no study has produced both LP derived prechondrocytes and preosteoblasts from human pluripotent stem cells (hPSC) through a common progenitor in a chemically defined manner. Differentiation of hPSCs through the authentic route, via an LP-osteochondral progenitor (OCP), may aid understanding of human cartilage development and the generation of effective cell therapies for osteoarthritis. We refined our existing chondrogenic protocol, incorporating knowledge from development and other studies to produce a LP-OCP from which prechondrocyte- and preosteoblast-like cells can be generated. Results show the formation of an OCP, which can be further driven to prechondrocytes and preosteoblasts. Prechondrocytes cultured in pellets produced cartilage like matrix with lacunae and superficial flattened cells expressing lubricin. Additionally, preosteoblasts were able to generate a mineralised structure. This protocol can therefore be used to investigate further cartilage development and in the development of joint cartilage for potential treatments.
Identifiants
pubmed: 36706111
doi: 10.1371/journal.pone.0280024
pii: PONE-D-22-15771
pmc: PMC9882893
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0280024Subventions
Organisme : Versus Arthritis
ID : 20786
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/T016744/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N013751/11
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/L004992/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P010709/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/L011840/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/K019392/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S002553/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : G0801057
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/K026666
Pays : United Kingdom
Informations de copyright
Copyright: © 2023 Smith et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
No, there is no conflict of interest. My manuscript contains the following statement: "The authors declare that they have no conflict of interest."
Références
Stem Cells Transl Med. 2017 Jan;6(1):40-50
pubmed: 28170184
Sci Rep. 2012;2:455
pubmed: 22701159
Lancet. 2019 Apr 27;393(10182):1745-1759
pubmed: 31034380
Stem Cell Reports. 2015 Mar 10;4(3):404-18
pubmed: 25733017
Mech Ageing Dev. 2008 Mar;129(3):163-73
pubmed: 18241911
Nat Commun. 2017 May 16;8:15040
pubmed: 28508891
Cell Res. 2021 Jul;31(7):742-757
pubmed: 33473154
Cell Stem Cell. 2018 Sep 6;23(3):382-395.e5
pubmed: 30100166
Stem Cells. 2007 Jan;25(1):29-38
pubmed: 17204604
Development. 2005 Mar;132(5):873-84
pubmed: 15673572
Cell Stem Cell. 2011 Aug 5;9(2):144-55
pubmed: 21816365
Cell Tissue Res. 1999 Apr;296(1):111-9
pubmed: 10199971
Stem Cell Res. 2020 Apr;44:101738
pubmed: 32109723
Birth Defects Res C Embryo Today. 2003 May;69(2):144-55
pubmed: 12955858
Cell Stem Cell. 2014 Sep 4;15(3):310-325
pubmed: 25042702
J Orthop Sci. 2004;9(3):270-3
pubmed: 15168182
Development. 2019 May 28;146(10):
pubmed: 31076486
Exp Mol Med. 2020 Aug;52(8):1166-1177
pubmed: 32788657
Stem Cell Reports. 2019 Sep 10;13(3):530-544
pubmed: 31402337
Science. 1998 Nov 6;282(5391):1145-7
pubmed: 9804556
Nat Biomed Eng. 2021 Aug;5(8):926-940
pubmed: 34373601
Stem Cell Reports. 2018 Mar 13;10(3):766-779
pubmed: 29429961
Nat Commun. 2021 Jan 13;12(1):362
pubmed: 33441552
Nat Biotechnol. 2010 Nov;28(11):1187-94
pubmed: 20967028
Nat Rev Rheumatol. 2014 Jul;10(7):437-41
pubmed: 24662640
EClinicalMedicine. 2020 Nov 26;29-30:100587
pubmed: 34505846
Cell. 2016 Jul 14;166(2):451-467
pubmed: 27419872
Nat Biomed Eng. 2019 Jul;3(7):558-570
pubmed: 31182836
Biomaterials. 2019 Mar;196:31-45
pubmed: 29456164
J Cell Sci. 2003 May 15;116(Pt 10):2015-28
pubmed: 12679385
Dev Biol. 1999 May 1;209(1):11-27
pubmed: 10208739
Nat Cell Biol. 2014 Jan;16(1):118-26
pubmed: 24335651
Am J Physiol Heart Circ Physiol. 2009 Jun;296(6):H1793-803
pubmed: 19363129
Matrix. 1991 Feb;11(1):17-24
pubmed: 2027327
Development. 2018 Mar 19;145(6):
pubmed: 29555813
Stem Cells Transl Med. 2014 Nov;3(11):1287-94
pubmed: 25273540
Cell Mol Life Sci. 2020 Jul;77(13):2543-2563
pubmed: 31915836
Development. 2015 Jun 15;142(12):2121-35
pubmed: 26015544
Regen Ther. 2020 Jan 14;14:19-31
pubmed: 31988991
Front Surg. 2015 Aug 13;2:39
pubmed: 26322314
Stem Cells Dev. 2013 Jul 1;22(13):1893-906
pubmed: 23413973
Scand J Clin Lab Invest. 2008;68(1):58-67
pubmed: 18224557
Development. 2014 Oct;141(20):3848-58
pubmed: 25294938
Osteoarthritis Cartilage. 2019 Mar;27(3):493-503
pubmed: 30502449
Nat Biotechnol. 2015 Jun;33(6):638-45
pubmed: 25961409
Nat Rev Rheumatol. 2019 Sep;15(9):550-570
pubmed: 31296933
FASEB J. 2022 May;36(5):e22314
pubmed: 35416346
J Cell Physiol. 2015 Feb;230(2):440-8
pubmed: 25078095
Biomater Res. 2018 Mar 16;22:9
pubmed: 29568558
J Tissue Eng Regen Med. 2011 Feb;5(2):146-50
pubmed: 20603892
Development. 2013 Jun;140(12):2597-610
pubmed: 23715552
Stem Cells. 2020 Sep;38(9):1107-1123
pubmed: 32442326
Stem Cell Res Ther. 2017 Jun 5;8(1):128
pubmed: 28583200
Cardiovasc Res. 2004 Aug 1;63(2):196-207
pubmed: 15249177
Stem Cell Res. 2019 Aug;39:101497
pubmed: 31326745