Gaseous nitric oxide failed to inhibit the replication cycle of SARS-CoV-2 in vitro.
COVID-19
Gaseous nitric oxide
SARS-CoV-2
Journal
Nitric oxide : biology and chemistry
ISSN: 1089-8611
Titre abrégé: Nitric Oxide
Pays: United States
ID NLM: 9709307
Informations de publication
Date de publication:
01 03 2023
01 03 2023
Historique:
received:
21
10
2022
revised:
22
01
2023
accepted:
23
01
2023
pubmed:
28
1
2023
medline:
16
2
2023
entrez:
27
1
2023
Statut:
ppublish
Résumé
Nitric oxide (NO) has been shown to have antimicrobial activity in vitro and in some in vivo models, while the virucidal activity of NO remains elusive. Some studies using NO donors have suggested that NO could be a potential candidate to treat SARS-CoV infection. The Covid-19 pandemic raised the hypothesis that NO gas might have an impact on Sars-CoV-2 replication cycle and might be considered as a candidate therapy to treat COVID-19. To our knowledge, there are no in vitro preclinical studies demonstrating a virucidal effect of gaseous NO on SARS-CoV-2. This study aims to determine whether gaseous NO has an impact on the replication cycle of SARS-CoV-2 in vitro. To that end, SARS-CoV-2 infected epithelial (VeroE6) and pulmonary (A549-hACE2) cells were treated with repeated doses of gaseous NO at different concentrations known to be efficient against bacteria. Our results show that exposing SARS-CoV-2 infected-cells to NO gas even at high doses (160 ppm, 6 h) does not influence the replication cycle of the virus in vitro. We report here that NO gas has no antiviral properties in vitro on SARS-COV-2. Therefore, there is no rationale for its usage in clinical settings to treat COVID-19 patients for direct antiviral purposes, which does not exclude other potential physiological benefits of this gas.
Identifiants
pubmed: 36706864
pii: S1089-8603(23)00010-1
doi: 10.1016/j.niox.2023.01.004
pmc: PMC9873364
pii:
doi:
Substances chimiques
Nitric Oxide
31C4KY9ESH
Antiviral Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
27-33Informations de copyright
Copyright © 2023. Published by Elsevier Inc.
Déclaration de conflit d'intérêts
Declaration of competing interest The study was funded by Air Liquide. AR, IK, JFRG and GF are employees of Air Liquide.
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