Persistence of birth mode-dependent effects on gut microbiome composition, immune system stimulation and antimicrobial resistance during the first year of life.
Journal
ISME communications
ISSN: 2730-6151
Titre abrégé: ISME Commun
Pays: England
ID NLM: 9918205372406676
Informations de publication
Date de publication:
26 Mar 2021
26 Mar 2021
Historique:
received:
27
10
2020
accepted:
02
01
2021
revised:
09
12
2020
entrez:
30
1
2023
pubmed:
26
3
2021
medline:
26
3
2021
Statut:
epublish
Résumé
Caesarean section delivery (CSD) disrupts mother-to-neonate transmission of specific microbial strains and functional repertoires as well as linked immune system priming. Here we investigate whether differences in microbiome composition and impacts on host physiology persist at 1 year of age. We perform high-resolution, quantitative metagenomic analyses of the gut microbiomes of infants born by vaginal delivery (VD) or by CSD, from immediately after birth through to 1 year of life. Several microbial populations show distinct enrichments in CSD-born infants at 1 year of age including strains of Bacteroides caccae, Bifidobacterium bifidum and Ruminococcus gnavus, whereas others are present at higher levels in the VD group including Faecalibacterium prausnitizii, Bifidobacterium breve and Bifidobacterium kashiwanohense. The stimulation of healthy donor-derived primary human immune cells with LPS isolated from neonatal stool samples results in higher levels of tumour necrosis factor alpha (TNF-α) in the case of CSD extracts over time, compared to extracts from VD infants for which no such changes were observed during the first year of life. Functional analyses of the VD metagenomes at 1 year of age demonstrate a significant increase in the biosynthesis of the natural antibiotics, carbapenem and phenazine. Concurrently, we find antimicrobial resistance (AMR) genes against several classes of antibiotics in both VD and CSD. The abundance of AMR genes against synthetic (including semi-synthetic) agents such as phenicol, pleuromutilin and diaminopyrimidine are increased in CSD children at day 5 after birth. In addition, we find that mobile genetic elements, including phages, encode AMR genes such as glycopeptide, diaminopyrimidine and multidrug resistance genes. Our results demonstrate persistent effects at 1 year of life resulting from birth mode-dependent differences in earliest gut microbiome colonisation.
Identifiants
pubmed: 36717704
doi: 10.1038/s43705-021-00003-5
pii: 10.1038/s43705-021-00003-5
pmc: PMC9723731
doi:
Types de publication
Journal Article
Langues
eng
Pagination
8Subventions
Organisme : Fonds National de la Recherche Luxembourg (National Research Fund)
ID : ATTRACT/A09/03
Organisme : Fonds National de la Recherche Luxembourg (National Research Fund)
ID : CORE/C15/SR/10404839
Organisme : Fonds National de la Recherche Luxembourg (National Research Fund)
ID : PRIDE17/11823097
Organisme : Fonds National de la Recherche Luxembourg (National Research Fund)
ID : AFR PHD-2013-5824125
Organisme : Fonds National de la Recherche Luxembourg (National Research Fund)
ID : CORE Junior/14/BM/8066232
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation)
ID : CRSII5_180241
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : FZT 118
Informations de copyright
© 2021. The Author(s).
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