Alterations of ATG4A and LC3B in neurons derived from Alzheimer's disease patients.
ATG4A
Alzheimer's disease
LC3B
amyloid β
autophagy
iPSC
Journal
Genes to cells : devoted to molecular & cellular mechanisms
ISSN: 1365-2443
Titre abrégé: Genes Cells
Pays: England
ID NLM: 9607379
Informations de publication
Date de publication:
Apr 2023
Apr 2023
Historique:
revised:
24
01
2023
received:
22
12
2022
accepted:
27
01
2023
medline:
5
4
2023
pubmed:
1
2
2023
entrez:
31
1
2023
Statut:
ppublish
Résumé
We investigated the alterations in autophagy-related molecules in neurons differentiated from induced pluripotent stem cells obtained from patients with Alzheimer's disease (AD). Consistent with our previous microarray data, ATG4A protein was upregulated in the neurons derived from a familial AD patient with an APP-E693Δ mutation who showed accumulation of intracellular amyloid β peptide (Aβ). This upregulation was reversed by inhibiting Aβ production, suggesting that the intracellular Aβ may be responsible for the upregulation of ATG4A. The LC3B-II/LC3B-I ratio, an index of autophagosome formation, was lower in the neurons derived from the AD patient with APP-E693Δ as well as the neurons derived from other familial and sporadic AD patients. These findings indicate that dysregulation of autophagy-related molecules may accelerate the pathogenesis of AD.
Substances chimiques
Amyloid beta-Peptides
0
ATG4A protein, human
EC 3.4.22.-
Autophagy-Related Proteins
0
Cysteine Endopeptidases
EC 3.4.22.-
MAP1LC3B protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
319-325Subventions
Organisme : Core Research for Evolutional Science and Technology
Informations de copyright
© 2023 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.
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