In vitro toxicological assessment of PhSeZnCl in human liver cells.
Apoptosis
Cell cycle
Comet assay
HepG2
HepaRG
Phenylselenenylzinc chloride
Journal
Toxicological research
ISSN: 1976-8257
Titre abrégé: Toxicol Res
Pays: Singapore
ID NLM: 101483324
Informations de publication
Date de publication:
Jan 2023
Jan 2023
Historique:
received:
17
09
2021
revised:
24
06
2022
accepted:
05
07
2022
entrez:
1
2
2023
pubmed:
2
2
2023
medline:
2
2
2023
Statut:
epublish
Résumé
Phenylselenenylzinc chloride (PhSeZnCl) is an air-stable selenolate, easily synthesizable through oxidative insertion of elemental zinc into the Se-halogen bond of the commercially available phenylselenyl chloride. PhSeZnCl was shown to possess a marked GPx-like activity both in NMR and in vitro tests, and to effectively react with cellular thiols, and was supposed for a potential use in the chemotherapy of drug-resistant cancers. However, activity of PhSeZnCl in hepatic cells has never been tested before now. In this in vitro approach, we evaluated the cytotoxic, genotoxic, and apoptotic activities, as well as the effects on cell cycle of PhSeZnCl in two preclinical hepatic models, namely HepG2 and HepaRG cells. Results showed that cell viability of HepG2 and HepaRG cells decreased in a dose-dependent manner, with a more marked effect in HepG2 tumour cells. Moreover, treatment with 50 µg/mL PhSeZnCl caused an increase of primary DNA damage (4 h) and a statistically significant increase of HepG2 cells arrested in G The online version contains supplementary material available at 10.1007/s43188-022-00148-y.
Identifiants
pubmed: 36721677
doi: 10.1007/s43188-022-00148-y
pii: 148
pmc: PMC9839901
doi:
Types de publication
Journal Article
Langues
eng
Pagination
105-114Informations de copyright
© The Author(s) 2022.
Déclaration de conflit d'intérêts
Conflict of interestThe authors declare that there is no conflict of interest regarding the publication of this paper.
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