Genome-wide characterization of mitochondrial DNA methylation in human brain.
5-Methylcytosine (5mC)
Brain
DNA Methylation
Mitochondria
epigenetics
mtDNA
Journal
Frontiers in endocrinology
ISSN: 1664-2392
Titre abrégé: Front Endocrinol (Lausanne)
Pays: Switzerland
ID NLM: 101555782
Informations de publication
Date de publication:
2022
2022
Historique:
received:
30
09
2022
accepted:
05
12
2022
entrez:
2
2
2023
pubmed:
3
2
2023
medline:
4
2
2023
Statut:
epublish
Résumé
There is growing interest in the role of DNA methylation in regulating the transcription of mitochondrial genes, particularly in brain disorders characterized by mitochondrial dysfunction. Here, we present a novel approach to interrogate the mitochondrial DNA methylome at single base resolution using targeted bisulfite sequencing. We applied this method to investigate mitochondrial DNA methylation patterns in post-mortem superior temporal gyrus and cerebellum brain tissue from seven human donors. We show that mitochondrial DNA methylation patterns are relatively low but conserved, with peaks in DNA methylation at several sites, such as within the We have annotated patterns of DNA methylation at single base resolution across the mitochondrial genome in human brain samples. Looking to the future this approach could be utilized to investigate the role of mitochondrial epigenetic mechanisms in disorders that display mitochondrial dysfunction.
Sections du résumé
Background
There is growing interest in the role of DNA methylation in regulating the transcription of mitochondrial genes, particularly in brain disorders characterized by mitochondrial dysfunction. Here, we present a novel approach to interrogate the mitochondrial DNA methylome at single base resolution using targeted bisulfite sequencing. We applied this method to investigate mitochondrial DNA methylation patterns in post-mortem superior temporal gyrus and cerebellum brain tissue from seven human donors.
Results
We show that mitochondrial DNA methylation patterns are relatively low but conserved, with peaks in DNA methylation at several sites, such as within the
Conclusions
We have annotated patterns of DNA methylation at single base resolution across the mitochondrial genome in human brain samples. Looking to the future this approach could be utilized to investigate the role of mitochondrial epigenetic mechanisms in disorders that display mitochondrial dysfunction.
Identifiants
pubmed: 36726473
doi: 10.3389/fendo.2022.1059120
pmc: PMC9885148
doi:
Substances chimiques
DNA, Mitochondrial
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1059120Subventions
Organisme : Medical Research Council
ID : MR/N027973/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : WT097835MF
Pays : United Kingdom
Organisme : Wellcome Trust
ID : WT101650MA
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/M008924/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/K003240/1
Pays : United Kingdom
Informations de copyright
Copyright © 2023 Devall, Soanes, Smith, Dempster, Smith, Burrage, Iatrou, Hannon, Troakes, Moore, O’Neill, Al-Sarraj, Schalkwyk, Mill, Weedon and Lunnon.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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