Descemet Stripping Only Technique for Corneal Endothelial Damage in Mice.
Journal
Cornea
ISSN: 1536-4798
Titre abrégé: Cornea
Pays: United States
ID NLM: 8216186
Informations de publication
Date de publication:
01 Apr 2023
01 Apr 2023
Historique:
received:
09
08
2022
accepted:
14
11
2022
pmc-release:
01
04
2024
pubmed:
3
2
2023
medline:
4
3
2023
entrez:
2
2
2023
Statut:
ppublish
Résumé
Descemet stripping only is an emerging surgical technique used to remove central Descemet membrane and corneal endothelial cells in patients with corneal endothelial disease. Here, we describe a murine model of this procedure to help facilitate basic science investigation and evaluation of postoperative outcomes using this surgical technique. Slitlamp biomicroscopy, central corneal thickness assessment (by optical coherence tomography), and immunohistochemistry were used to assess the model through 7 weeks of follow-up. Complete removal of the endothelium and Descemet membrane was confirmed by slitlamp biomicroscopy and by histology. Central corneal thickness peaked at day 1 postinjury and then declined over the course of 2 weeks to a stable level of persistent edema. Seven weeks postinjury, immunohistochemical staining for ZO-1 showed the area of Descemet stripping was fully covered by enlarged and dysmorphic corneal endothelial cell. No significant ocular complications were appreciated through the end of the follow-up. We demonstrate the feasibility of and provide detailed instructions for a murine model of Descemet stripping only. This model provides a potential in vivo platform to investigate the mechanisms and biology of this emerging surgical procedure.
Identifiants
pubmed: 36728991
doi: 10.1097/ICO.0000000000003223
pii: 00003226-990000000-00209
pmc: PMC10117527
mid: NIHMS1852219
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
470-475Subventions
Organisme : NEI NIH HHS
ID : K08 EY031759
Pays : United States
Informations de copyright
Copyright © 2022 Wolters Kluwer Health, Inc. All rights reserved.
Déclaration de conflit d'intérêts
The authors have no funding or conflicts of interest to disclose.
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