Clinical and MRI Evolution After Local Injection of Bone Marrow-Derived Mesenchymal Stem Cells in Perianal Fistulae in Crohn's Disease: Results From a Prospective Monocentric Study.


Journal

Journal of Crohn's & colitis
ISSN: 1876-4479
Titre abrégé: J Crohns Colitis
Pays: England
ID NLM: 101318676

Informations de publication

Date de publication:
03 May 2023
Historique:
medline: 5 5 2023
pubmed: 4 2 2023
entrez: 3 2 2023
Statut: ppublish

Résumé

Local injection of adipose tissue-derived mesenchymal stem cells [MSCs] is effective in fistulizing perianal Crohn's disease [CD]. Less is known about bone marrow-derived MSCs and little is known about predictive factors of response and magnetic resonance imaging [MRI] evolution of the fistulae after MSC injection. Our aims were to evaluate the safety and clinical outcome of bone marrow-derived MSC injection for perianal fistulizing CD, to evaluate the MRI evolution of the fistulae and to identify factors associated with fistula closure. All CD patients with perianal fistula and appropriate drainage with a seton without abscess at MRI were eligible. Clinical examination, biomarkers and pelvic MRI were performed at weeks 0, 12 and 48. The clinical outcome was assessed by closure of the treated external openings at clinical examination and MRI exploration. Sixteen patients with a median age of 49 years and a median duration of perianal CD of 8 months were included. No unexpected safety event occurred. At weeks 12 and 48, 9/16 and 8/16 patients had complete fistula[e] closure, respectively, whereas 11/16 patients had at least partial closure. At MRI, the degree of fibrosis increased significantly after MSC injection. In total, 86% of patients with >80% of fibrosis of the fistula tract at week 48 had fistula closure. Fistula closure at week 12 was predictive of fistula closure at week 48. The MAGNIFI-CD did not change significantly over time. Open-label injection of bone marrow-derived MSCs was safe and was effective in half of the patients in fistulizing perianal CD and induced significant MRI changes associated with favourable clinical outcome.

Sections du résumé

BACKGROUND BACKGROUND
Local injection of adipose tissue-derived mesenchymal stem cells [MSCs] is effective in fistulizing perianal Crohn's disease [CD]. Less is known about bone marrow-derived MSCs and little is known about predictive factors of response and magnetic resonance imaging [MRI] evolution of the fistulae after MSC injection. Our aims were to evaluate the safety and clinical outcome of bone marrow-derived MSC injection for perianal fistulizing CD, to evaluate the MRI evolution of the fistulae and to identify factors associated with fistula closure.
PATIENTS AND METHODS METHODS
All CD patients with perianal fistula and appropriate drainage with a seton without abscess at MRI were eligible. Clinical examination, biomarkers and pelvic MRI were performed at weeks 0, 12 and 48. The clinical outcome was assessed by closure of the treated external openings at clinical examination and MRI exploration.
RESULTS RESULTS
Sixteen patients with a median age of 49 years and a median duration of perianal CD of 8 months were included. No unexpected safety event occurred. At weeks 12 and 48, 9/16 and 8/16 patients had complete fistula[e] closure, respectively, whereas 11/16 patients had at least partial closure. At MRI, the degree of fibrosis increased significantly after MSC injection. In total, 86% of patients with >80% of fibrosis of the fistula tract at week 48 had fistula closure. Fistula closure at week 12 was predictive of fistula closure at week 48. The MAGNIFI-CD did not change significantly over time.
CONCLUSION CONCLUSIONS
Open-label injection of bone marrow-derived MSCs was safe and was effective in half of the patients in fistulizing perianal CD and induced significant MRI changes associated with favourable clinical outcome.

Identifiants

pubmed: 36733215
pii: 7025057
doi: 10.1093/ecco-jcc/jjac192
doi:

Types de publication

Clinical Trial Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

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Informations de copyright

© The Author(s) 2023. Published by Oxford University Press on behalf of European Crohn’s and Colitis Organisation. All rights reserved. For permissions, please email: journals.permissions@oup.com.

Auteurs

Catherine Reenaers (C)

Gastroenterology Department, CHU Sart-Tilman, Liège, Belgium.

Romain P Gillard (RP)

Radiology Department, CHU Sart-Tilman, Liège, Belgium.

Carla Coimbra (C)

Abdominal Surgery Department, CHU Sart-Tilman, Liège, Belgium.

René M Gillard (RM)

Radiology Department, CHU Sart-Tilman, Liège, Belgium.

Paul Meunier (P)

Radiology Department, CHU Sart-Tilman, Liège, Belgium.

Chantal Lechanteur (C)

University of Liège, Laboratory of Cell and Gene Therapy LTCG, Liège, Belgium.

Etienne Baudoux (E)

University of Liège, Laboratory of Cell and Gene Therapy LTCG, Liège, Belgium.

Layla Boutaffala (L)

Gastroenterology Department, CHU Sart-Tilman, Liège, Belgium.

Yves Beguin (Y)

University of Liège, Laboratory of Cell and Gene Therapy LTCG, Liège, Belgium.
Hematology Department, CHU Sart-Tilman, Liège, Belgium.

Édouard Louis (É)

Gastroenterology Department, CHU Sart-Tilman, Liège, Belgium.

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