Spatial analysis of the ancient proteome of archeological teeth using mass spectrometry imaging.
Journal
Rapid communications in mass spectrometry : RCM
ISSN: 1097-0231
Titre abrégé: Rapid Commun Mass Spectrom
Pays: England
ID NLM: 8802365
Informations de publication
Date de publication:
30 Apr 2023
30 Apr 2023
Historique:
revised:
28
01
2023
received:
13
11
2022
accepted:
29
01
2023
pubmed:
4
2
2023
medline:
16
3
2023
entrez:
3
2
2023
Statut:
ppublish
Résumé
Proteins extracted from archaeological bone and teeth are utilised for investigating the phylogeny of extinct and extant species, the biological sex and age of past individuals, as well as ancient health and physiology. However, variable preservation of proteins in archaeological materials represents a major challenge. To better understand the spatial distribution of ancient proteins preserved within teeth, we applied matrix assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) for the first time to bioarchaeological samples to visualise the intensity of proteins in archaeological teeth thin sections. We specifically explored the spatial distribution of four proteins (collagen type I, of which the chains alpha-1 and alpha-2, alpha-2-HS-glycoprotein, haemoglobin subunit alpha and myosin light polypeptide 6). We successfully identified ancient proteins in archaeological teeth thin sections using mass spectrometry imaging. The data are available via ProteomeXchange with identifier PXD038114. However, we observed that peptides did not always follow our hypotheses for their spatial distribution, with distinct differences observed in the spatial distribution of several proteins, and occasionally between peptides of the same protein. While it remains unclear what causes these differences in protein intensity distribution within teeth, as revealed by MALDI-MSI in this study, we have demonstrated that MALDI-MSI can be successfully applied to mineralised bioarchaeological tissues to detect ancient peptides. In future applications, this technique could be particularly fruitful not just for understanding the preservation of proteins in a range of archaeological materials, but making informed decisions on sampling strategies and the targeting of key proteins of archaeological and biological interest.
Substances chimiques
Proteome
0
Peptides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e9486Subventions
Organisme : Leverhulme Trust
ID : Philip Leverhulme Prize
Organisme : Nederlandse Organisatie voor Wetenschappelijk Onderzoek
ID : Neanderthal Legacy
Organisme : Nederlandse Organisatie voor Wetenschappelijk Onderzoek
ID : VI.C.191.07
Organisme : University of York
ID : Research Priming Fund
Informations de copyright
© 2023 The Authors. Rapid Communications in Mass Spectrometry published by John Wiley & Sons Ltd.
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