Multimodal human thymic profiling reveals trajectories and cellular milieu for T agonist selection.
T agonist selection
T cell development
antigen-presenting cells
autoimmunity
human thymus
multi-modal
single-cell RNA sequencing
spatial transcriptomics
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2022
2022
Historique:
received:
07
11
2022
accepted:
22
12
2022
entrez:
6
2
2023
pubmed:
7
2
2023
medline:
8
2
2023
Statut:
epublish
Résumé
To prevent autoimmunity, thymocytes expressing self-reactive T cell receptors (TCRs) are negatively selected, however, divergence into tolerogenic, agonist selected lineages represent an alternative fate. As thymocyte development, selection, and lineage choices are dependent on spatial context and cell-to-cell interactions, we have performed Cellular Indexing of Transcriptomes and Epitopes by sequencing (CITE-seq) and spatial transcriptomics on paediatric human thymus. Thymocytes expressing markers of strong TCR signalling diverged from the conventional developmental trajectory prior to CD4
Identifiants
pubmed: 36741401
doi: 10.3389/fimmu.2022.1092028
pmc: PMC9895842
doi:
Substances chimiques
Receptors, Antigen, T-Cell
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1092028Informations de copyright
Copyright © 2023 Heimli, Flåm, Hjorthaug, Trinh, Frisk, Dumont, Ribarska, Tekpli, Saare and Lie.
Déclaration de conflit d'intérêts
TR was employed by the company Exact Sciences Innovation Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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