Exploiting photosynthesis-driven P450 activity to produce indican in tobacco chloroplasts.
chloroplast
cytochrome P450
ferredoxin
indigo
metabolic engineering
photosynthesis
transient expression
Journal
Frontiers in plant science
ISSN: 1664-462X
Titre abrégé: Front Plant Sci
Pays: Switzerland
ID NLM: 101568200
Informations de publication
Date de publication:
2022
2022
Historique:
received:
20
09
2022
accepted:
14
12
2022
entrez:
6
2
2023
pubmed:
7
2
2023
medline:
7
2
2023
Statut:
epublish
Résumé
Photosynthetic organelles offer attractive features for engineering small molecule bioproduction by their ability to convert solar energy into chemical energy required for metabolism. The possibility to couple biochemical production directly to photosynthetic assimilation as a source of energy and substrates has intrigued metabolic engineers. Specifically, the chemical diversity found in plants often relies on cytochrome P450-mediated hydroxylations that depend on reductant supply for catalysis and which often lead to metabolic bottlenecks for heterologous production of complex molecules. By directing P450 enzymes to plant chloroplasts one can elegantly deal with such redox prerequisites. In this study, we explore the capacity of the plant photosynthetic machinery to drive P450-dependent formation of the indigo precursor indoxyl-β-D-glucoside (indican) by targeting an engineered indican biosynthetic pathway to tobacco (
Identifiants
pubmed: 36743583
doi: 10.3389/fpls.2022.1049177
pmc: PMC9890960
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1049177Informations de copyright
Copyright © 2023 Mellor, Behrendorff, Ipsen, Crocoll, Laursen, Gillam and Pribil.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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