Molecular characterization of the intact mouse muscle spindle using a multi-omics approach.
connective tissue
developmental biology
intrafusal fibers
mouse
muscle spindle
proprioception
skeletal deformation
γ-motoneurons
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
06 02 2023
06 02 2023
Historique:
received:
13
07
2022
accepted:
03
02
2023
pubmed:
7
2
2023
medline:
18
2
2023
entrez:
6
2
2023
Statut:
epublish
Résumé
The proprioceptive system is essential for the control of coordinated movement, posture, and skeletal integrity. The sense of proprioception is produced in the brain using peripheral sensory input from receptors such as the muscle spindle, which detects changes in the length of skeletal muscles. Despite its importance, the molecular composition of the muscle spindle is largely unknown. In this study, we generated comprehensive transcriptomic and proteomic datasets of the entire muscle spindle isolated from the murine deep masseter muscle. We then associated differentially expressed genes with the various tissues composing the spindle using bioinformatic analysis. Immunostaining verified these predictions, thus establishing new markers for the different spindle tissues. Utilizing these markers, we identified the differentiation stages the spindle capsule cells undergo during development. Together, these findings provide comprehensive molecular characterization of the intact spindle as well as new tools to study its development and function in health and disease.
Identifiants
pubmed: 36744866
doi: 10.7554/eLife.81843
pii: 81843
pmc: PMC9931388
doi:
pii:
Banques de données
GEO
['GSE208147', 'GSE161621', 'GSE131230']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2023, Bornstein, Heinemann-Yerushalmi et al.
Déclaration de conflit d'intérêts
BB, LH, SK, RA, BD, DL, MK, GB, RB, EZ No competing interests declared
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