Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration.
GLICO
cerebral organoids
glioblastoma
induced pluripotent stem cells
Journal
Molecular oncology
ISSN: 1878-0261
Titre abrégé: Mol Oncol
Pays: United States
ID NLM: 101308230
Informations de publication
Date de publication:
04 2023
04 2023
Historique:
revised:
22
01
2023
received:
05
05
2022
accepted:
03
02
2023
medline:
31
3
2023
pubmed:
7
2
2023
entrez:
6
2
2023
Statut:
ppublish
Résumé
It is currently challenging to adequately model the growth and migration of glioblastoma using two-dimensional (2D) in vitro culture systems as they quickly lose the original, patient-specific identity and heterogeneity. However, with the advent of three-dimensional (3D) cell cultures and human-induced pluripotent stem cell (iPSC)-derived cerebral organoids (COs), studies demonstrate that the glioblastoma-CO (GLICO) coculture model helps to preserve the phenotype of the patient-specific tissue. Here, we aimed to set up such a model using mature COs and develop a pipeline for subsequent analysis of cocultured glioblastoma. Our data demonstrate that the growth and migration of the glioblastoma cell line within the mature COs are significantly increased in the presence of extracellular matrix proteins, shortening the time needed for glioblastoma to initiate migration. We also describe in detail the method for the visualization and quantification of these migrating cells within the GLICO model. Lastly, we show that this coculture model (and the human brain-like microenvironment) can significantly transform the gene expression profile of the established U87 glioblastoma cell line into proneural and classical glioblastoma cell types.
Identifiants
pubmed: 36744875
doi: 10.1002/1878-0261.13389
pmc: PMC10061278
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
647-663Subventions
Organisme : Grantová Agentura České Republiky
ID : 21-21510S
Organisme : Grantová Agentura České Republiky
ID : 20-15728S
Organisme : Grantová Agentura České Republiky
ID : 18-25429Y
Organisme : AZV
ID : NV19-08-00472
Organisme : AZV
ID : NU21-08-00373
Organisme : Grant Agency of Masaryk University
ID : MUNI/11/SUP/19/2020
Organisme : Grant Agency of Masaryk University
ID : MUNI/11/SUP/01/2022
Organisme : Grant Agency of Masaryk University
ID : MUNI/A/1398/2021
Organisme : European Regional Development Fund - Project INBIO
ID : CZ.02.1.01/0.0/0.0/16_026/0008451
Organisme : RVO
ID : 86652036
Organisme : Career Restart Grant
ID : MUNI/R/1321/2021
Organisme : Career Restart Grant
ID : MUNI/R/1697/2020
Organisme : Medical Faculty MU
ID : ROZV/28/LF22/2020
Organisme : Medical Faculty MU
ID : ROZV/23/LF19/2019
Organisme : JCMM - PhD Talent
ID : MUNI/IGA/1273/2021
Organisme : MEYS CR
ID : LQ1605
Organisme : MEYS CR
ID : CZ.02.1.01/0.0/0.0/16_013/0001761
Organisme : MEYS CR
ID : LM2018121
Organisme : MEYS CR
ID : LM2018132
Organisme : MEYS CR
ID : LM2018129
Organisme : European Union's Horizon 2020 Research and Innovation Program
ID : 857560
Organisme : Operational Programme Research, Development and Innovation - project CETOCOEN EXCELLENCE
ID : CZ.02.1.01/0.0/0.0/17_043/0009632
Informations de copyright
© 2023 The Authors. Molecular Oncology published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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