Crystalline Antibody-Laden Alginate Particles: A Platform for Enabling High Concentration Subcutaneous Delivery of Antibodies.


Journal

Advanced healthcare materials
ISSN: 2192-2659
Titre abrégé: Adv Healthc Mater
Pays: Germany
ID NLM: 101581613

Informations de publication

Date de publication:
06 2023
Historique:
revised: 30 01 2023
received: 15 09 2022
medline: 15 6 2023
pubmed: 7 2 2023
entrez: 6 2 2023
Statut: ppublish

Résumé

Subcutaneous (SC) administration is a desired route for monoclonal antibodies (mAbs). However, formulating mAbs for small injection volumes at high concentrations with suitable stability and injectability is a significant challenge. Here, this work presents a platform technology that combines the stability of crystalline antibodies with injectability and tunability of soft hydrogel particles. Composite alginate hydrogel particles are generated via a gentle centrifugal encapsulation process which avoids use of chemical reactions or an external organic phase. Crystalline suspension of anti-programmed cell death protein 1 (PD-1) antibody (pembrolizumab) is utilized as a model therapeutic antibody. Crystalline forms of the mAb encapsuled in the hydrogel particles lead to stable, high concentration, and injectable formulations. Formulation concentrations as high as 315 mg mL

Identifiants

pubmed: 36745878
doi: 10.1002/adhm.202202370
doi:

Substances chimiques

Alginates 0
Antibodies, Monoclonal 0
Hydrogels 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2202370

Informations de copyright

© 2023 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.

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Auteurs

Amir Erfani (A)

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.

Jeremy M Schieferstein (JM)

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.

Paul Reichert (P)

Merck Research Laboratories, Kenilworth, NJ, 07033, USA.

Chakravarthy N Narasimhan (CN)

Merck Research Laboratories, Kenilworth, NJ, 07033, USA.

Cinthia Pastuskovas (C)

Merck Research Laboratories, Kenilworth, NJ, 07033, USA.

Vaishali Parab (V)

Merck Research Laboratories, Kenilworth, NJ, 07033, USA.

Denarra Simmons (D)

Merck Research Laboratories, Kenilworth, NJ, 07033, USA.

Xiaoyu Yang (X)

Merck Research Laboratories, Kenilworth, NJ, 07033, USA.

Apoorv Shanker (A)

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.
Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.

Paula Hammond (P)

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.
Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.

Patrick S Doyle (PS)

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.
Harvard Medical School Initiative for RNA Medicine, Boston, MA, 02215, USA.

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