Kinetics of viral DNA in body fluids and antibody response in patients with acute Monkeypox virus infection.
Immunology
Virology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
17 Mar 2023
17 Mar 2023
Historique:
received:
24
10
2022
revised:
18
11
2022
accepted:
26
01
2023
pubmed:
8
2
2023
medline:
8
2
2023
entrez:
7
2
2023
Statut:
ppublish
Résumé
We report the follow-up laboratory investigation of three MPXV cases infected in May-June 2022 from diagnosis to disease resolution, monitoring viral shedding in different body fluids and antibody kinetics. Out of 138 non-lesion samples, viral DNA was found in 92.3% saliva, 85.7% semen, 86.2% oropharyngeal swabs, 51.7% plasma, 46.1% stool, and 9.5% urine samples. Viral load quantified by digital PCR widely varied, but tend to be higher in oropharyngeal swabs, saliva, and stool. Replication competent virus was recovered from four out of seventeen samples, including 1 saliva, 1 oropharyngeal swabs, 1 semen, and 1 stool. The analysis of the antibody kinetics revealed that IgM, IgA, and IgG antibodies were detected within two weeks post-symptoms onset for all three patients, with IgG detected early on at day 4-8 and IgM and IgA showing lower titers along the time frame of the study. Antibody levels increased during the second week of illness with IgG reaching high titers.
Identifiants
pubmed: 36748085
doi: 10.1016/j.isci.2023.106102
pii: S2589-0042(23)00179-7
pmc: PMC9893533
doi:
Types de publication
Journal Article
Langues
eng
Pagination
106102Informations de copyright
© 2023 The Authors.
Déclaration de conflit d'intérêts
The authors declare that no conflicting financial interests or other competing relationships exist for the present study.
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