Microglial motility is modulated by neuronal activity and correlates with dendritic spine plasticity in the hippocampus of awake mice.
chemogenetics
dendritic spines
hippocampus
microglia
mouse
neuroscience
two-photon
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
07 02 2023
07 02 2023
Historique:
received:
01
09
2022
accepted:
03
02
2023
pubmed:
8
2
2023
medline:
25
2
2023
entrez:
7
2
2023
Statut:
epublish
Résumé
Microglia, the resident immune cells of the brain, play a complex role in health and disease. They actively survey the brain parenchyma by physically interacting with other cells and structurally shaping the brain. Yet, the mechanisms underlying microglial motility and significance for synapse stability, especially in the hippocampus during adulthood, remain widely unresolved. Here, we investigated the effect of neuronal activity on microglial motility and the implications for the formation and survival of dendritic spines on hippocampal CA1 neurons in vivo. We used repetitive two-photon in vivo imaging in the hippocampus of awake and anesthetized mice to simultaneously study the motility of microglia and their interaction with dendritic spines. We found that CA3 to CA1 input is sufficient to modulate microglial process motility. Simultaneously, more dendritic spines emerged in mice after awake compared to anesthetized imaging. Interestingly, the rate of microglial contacts with individual dendritic spines and dendrites was associated with the stability, removal, and emergence of dendritic spines. These results suggest that microglia might sense neuronal activity via neurotransmitter release and actively participate in synaptic rewiring of the hippocampal neural network during adulthood. Further, this study has profound relevance for hippocampal learning and memory processes.
Identifiants
pubmed: 36749020
doi: 10.7554/eLife.83176
pii: 83176
pmc: PMC9946443
doi:
pii:
Banques de données
Dryad
['10.5061/dryad.63xsj3v68']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2023, Nebeling et al.
Déclaration de conflit d'intérêts
FN, SP, LJ, JS, KK, MM, MF No competing interests declared
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