Fragile X-Related Protein FXR1 Controls Human Adenovirus Capsid mRNA Metabolism.
FXR1
adenovirus
m6A-modification
mRNA decay
mRNA translation
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
28 02 2023
28 02 2023
Historique:
pubmed:
8
2
2023
medline:
3
3
2023
entrez:
7
2
2023
Statut:
ppublish
Résumé
Human adenoviruses (HAdVs) are widespread pathogens causing a variety of diseases. A well-controlled expression of virus capsid mRNAs originating from the major late transcription unit (MLTU) is essential for forming the infectious virus progeny. However, regulation of the MLTU mRNA metabolism has mainly remained enigmatic. In this study, we show that the cellular RNA-binding protein FXR1 controls the stability of the HAdV-5 MLTU mRNAs, as depletion of FXR1 resulted in increased steady-state levels of MLTU mRNAs. Surprisingly, the lack of FXR1 reduced viral capsid protein accumulation and formation of the infectious virus progeny, indicating an opposing function of FXR1 in HAdV-5 infection. Further, the long FXR1 isoform interfered with MLTU mRNA translation, suggesting FXR1 isoform-specific functions in virus-infected cells. We also show that the FXR1 protein interacts with N6-methyladenosine (m
Identifiants
pubmed: 36749074
doi: 10.1128/jvi.01539-22
pmc: PMC9972981
doi:
Substances chimiques
Capsid Proteins
0
FXR1 protein, human
0
RNA, Messenger
0
RNA-Binding Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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