Transient depletion of macrophages alters local inflammatory response at the site of disc herniation in a transgenic mouse model.
Back pain
Inflammation
Intervertebral disc herniation
Macrophage
Macrophage Fas-induced apoptosis (MaFIA)
Journal
Osteoarthritis and cartilage
ISSN: 1522-9653
Titre abrégé: Osteoarthritis Cartilage
Pays: England
ID NLM: 9305697
Informations de publication
Date de publication:
Jul 2023
Jul 2023
Historique:
received:
09
05
2022
revised:
06
10
2022
accepted:
10
01
2023
pmc-release:
01
07
2024
medline:
19
6
2023
pubmed:
9
2
2023
entrez:
8
2
2023
Statut:
ppublish
Résumé
Macrophages are abundantly detected at sites of disc herniation, however, their function in the disease progression is unclear. We aim to investigate the functions of macrophages in acute disc herniation using a macrophage Fas-induced apoptosis (MaFIA) transgenic mouse strain. To transiently deplete macrophages, a dimerizer, AP20187, or vehicle solution was administered via intraperitoneal injection to MaFIA mice immediately, day 1 and 2 after annular puncture induced disc herniation. Local infiltrated tissues at disc hernia and DRGs at corresponding levels were harvested to analyze immune cells and neuroinflammation on postoperative day (POD) 6 by flow cytometry and/or immunostaining. Mouse spines were harvested to analyze structures of degenerated discs and adjacent vertebrae and to assess osteoclast activity by histology and tartrate-resistant acid phosphatase (TRAP) staining on POD 6, 13, and 20, respectively. On POD 6, abundant macrophages were confirmed at disc hernia sites. Compared to vehicle control, AP20187 significantly reduced GFP Transient depletion of macrophages altered local inflammatory response at the site of disc herniation.
Identifiants
pubmed: 36754251
pii: S1063-4584(23)00593-9
doi: 10.1016/j.joca.2023.01.574
pmc: PMC10272080
mid: NIHMS1877831
pii:
doi:
Substances chimiques
AP20187
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
894-907Subventions
Organisme : NIAMS NIH HHS
ID : R21 AR082052
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR078888
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR064792
Pays : United States
Organisme : NIAMS NIH HHS
ID : R21 AR078547
Pays : United States
Organisme : NIAMS NIH HHS
ID : R21 AR072334
Pays : United States
Informations de copyright
Copyright © 2023 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors have no competing interests to declare.
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