Mode of autophosphorylation in bacteriophytochromes RpBphP2 and RpBphP3.
Autophosphorylation
Bacteriophytochrome
Crystal structure
Kinase activation
Photoreceptor
Sensory histidine kinase
Journal
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
ISSN: 1474-9092
Titre abrégé: Photochem Photobiol Sci
Pays: England
ID NLM: 101124451
Informations de publication
Date de publication:
Jun 2023
Jun 2023
Historique:
received:
29
11
2022
accepted:
12
01
2023
medline:
3
7
2023
pubmed:
10
2
2023
entrez:
9
2
2023
Statut:
ppublish
Résumé
Phytochromes are red-light photoreceptors that regulate a wide range of physiological processes in plants, fungi and bacteria. Canonical bacteriophytochromes are photosensory histidine kinases that undergo light-dependent autophosphorylation, thereby regulating cellular responses to red light via two-component signaling pathways. However, the molecular mechanism of kinase activation remains elusive for bacteriophytochromes. In particular, the directionality of autophosphorylation is still an open question in these dimeric photoreceptor kinases. In this work, we perform histidine kinase assays on two tandem bacteriophytochromes RpBphP2 and RpBphP3 from the photosynthetic bacterium Rhodopseudomonas palustris. By examining the kinase activities of full-length bacteriophytochromes and two loss-of-function mutants under different light conditions, we demonstrate that RpBphP2 and RpBphP3 undergo light-dependent trans-phosphorylation between protomers in both homodimeric and heterodimeric forms. We have further determined the crystal structure of the histidine kinase domains of RpBphP2 at 3.19 Å resolution. Based on structural comparisons and homology modeling, we also present a model to account for the actions of trans-autophosphorylation in bacteriophytochromes.
Identifiants
pubmed: 36757561
doi: 10.1007/s43630-023-00366-9
pii: 10.1007/s43630-023-00366-9
pmc: PMC10619329
mid: NIHMS1935658
doi:
Substances chimiques
Histidine Kinase
EC 2.7.13.1
Phytochrome
11121-56-5
Bacterial Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1257-1266Subventions
Organisme : NEI NIH HHS
ID : R01 EY024363
Pays : United States
Informations de copyright
© 2023. The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology.
Références
J Biol Chem. 2005 Sep 16;280(37):32389-97
pubmed: 16009707
Chemphyschem. 2010 Apr 26;11(6):1172-80
pubmed: 20155775
J Synchrotron Radiat. 2004 Jan 1;11(Pt 1):49-52
pubmed: 14646132
Annu Rev Plant Biol. 2006;57:837-58
pubmed: 16669784
Acta Crystallogr F Struct Biol Commun. 2015 Oct;71(Pt 10):1215-22
pubmed: 26457509
Cell. 2009 Oct 16;139(2):325-36
pubmed: 19800110
Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14715-20
pubmed: 18799746
Mol Microbiol. 2017 Jan;103(2):197-202
pubmed: 27618209
Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2010-E2019
pubmed: 29440402
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
Nature. 2005 Nov 17;438(7066):325-31
pubmed: 16292304
Elife. 2016 Dec 12;5:
pubmed: 27938660
mBio. 2021 Apr 20;12(2):
pubmed: 33879593
J Biol Chem. 2015 Jun 26;290(26):16383-92
pubmed: 25971964
Proc Natl Acad Sci U S A. 2007 Jul 24;104(30):12571-6
pubmed: 17640891
Nature. 2001 Dec 13;414(6865):776-9
pubmed: 11742406
Annu Rev Microbiol. 2012;66:325-47
pubmed: 22746333
Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16185-90
pubmed: 19805278
Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):17839-44
pubmed: 25468971
Photochem Photobiol. 2019 Nov;95(6):1369-1375
pubmed: 31230349
Nat Methods. 2022 Jun;19(6):679-682
pubmed: 35637307
PLoS Biol. 2014 Jan 28;12(1):e1001776
pubmed: 24492262
Nature. 2011 Oct 16;479(7373):428-32
pubmed: 22002602
J Bacteriol. 1993 Nov;175(21):7024-32
pubmed: 8226644
Structure. 2013 Jul 2;21(7):1127-36
pubmed: 23746806
Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11057-61
pubmed: 1662380
Methods Enzymol. 1997;276:307-26
pubmed: 27754618
Nat Commun. 2014;5:3258
pubmed: 24500224
Mol Cell Proteomics. 2006 Apr;5(4):749-57
pubmed: 16340016
Methods Enzymol. 1997;276:523-530
pubmed: 27799112
Science. 1997 Sep 5;277(5331):1505-8
pubmed: 9278513
Nature. 2021 Aug;596(7873):583-589
pubmed: 34265844
Structure. 2016 Mar 1;24(3):448-57
pubmed: 26853942
Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14709-14
pubmed: 18799745
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
Nucleic Acids Res. 2014 Jul;42(Web Server issue):W252-8
pubmed: 24782522
Structure. 2015 Jul 7;23(7):1179-89
pubmed: 26095026
Proc Natl Acad Sci U S A. 2019 Mar 12;116(11):4963-4972
pubmed: 30808807
Structure. 2015 Jun 2;23(6):981-94
pubmed: 25982528
Proc Natl Acad Sci U S A. 2019 Oct 1;116(40):19973-19982
pubmed: 31527275
J Biol Chem. 2020 Jun 5;295(23):8106-8117
pubmed: 32094228