Performance in (Ultra-)high-performance liquid chromatography-How to qualify and optimize instruments in practice.
extra column band broadening
liquid chromatography
superficially porous particles
system optimization
van Deemter curve
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Apr 2023
Apr 2023
Historique:
revised:
11
01
2023
received:
31
10
2022
accepted:
03
02
2023
medline:
10
2
2023
pubmed:
10
2
2023
entrez:
9
2
2023
Statut:
ppublish
Résumé
This paper investigates the suitability of an ultra-high-performance liquid chromatography/high-performance liquid chromatography hybrid system for ultra-high-performance liquid chromatography applications. Thus, the effect of extra column band broadening, the gradient system, and the injection system were tested and optimized according to their capabilities. An increase of the theoretical plate number up to a factor of two is achieved by the optimization of the extra column volume into the typical ultra-high-performance liquid chromatography range (<10 μl). Moreover, for qualitative purposes injections of volumes typical for ultra-high-performance liquid chromatography methods are precise. Despite this, a lack of precision and accuracy was determined for the gradient system, and the dwell volume meets the typical specification range for conventional HPLC systems. Therefore, hybrid systems are the intercept between both spectra and are limitedly suitable for ultra-high-performance liquid chromatography applications. Another way to approximate ultra-high-performance liquid chromatography performance using a high-performance liquid chromatography system is superficially porous particles. Thus, H/u curves of 5 μm superficially porous and 3 μm fully porous particles were recorded in order to determine the effect of the particle technology resulting in comparable performance of the used stationary phases.
Identifiants
pubmed: 36757818
doi: 10.1002/jssc.202200894
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2200894Informations de copyright
© 2023 Wiley-VCH GmbH.
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