Turnover of the extracellular polymeric matrix of granules performing biological phosphate removal.
13C incorporation study
Biofilm
Enhanced biological phosphate removal
Extracellular polymeric substances
Polyphosphate accumulating organisms
Protein and carbohydrate turnover
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Mar 2023
Mar 2023
Historique:
received:
16
08
2022
accepted:
31
01
2023
revised:
14
11
2022
pubmed:
10
2
2023
medline:
15
3
2023
entrez:
9
2
2023
Statut:
ppublish
Résumé
Polyphosphate accumulating organisms (PAOs) are responsible for enhanced biological phosphate removal (EBPR) from wastewater, where they grow embedded in a matrix of extracellular polymeric substances (EPS). EPSs comprise a mixture of biopolymers like polysaccharides or (glyco)proteins. Despite previous studies, little is known about the dynamics of EPS in mixed cultures, and their production by PAOs and potential consumption by flanking microbes. EPSs are biodegradable and have been suggested to be a substrate for other organisms in the community. Studying EPS turnover can help elucidate their biosynthesis and biodegradation cycles. We analyzed the turnover of proteins and polysaccharides in the EPS of an enrichment culture of PAOs relative to the turnover of internal proteins. An anaerobic-aerobic sequencing batch reactor (SBR) simulating EBPR conditions was operated to enrich for PAOs. After achieving a stable culture, carbon source was switched to uniformly
Identifiants
pubmed: 36759376
doi: 10.1007/s00253-023-12421-7
pii: 10.1007/s00253-023-12421-7
pmc: PMC10006046
doi:
Substances chimiques
Phosphorus
27YLU75U4W
Wastewater
0
Polyphosphates
0
Polymers
0
Sugars
0
Sewage
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1997-2009Subventions
Organisme : Nederlandse Organisatie voor Wetenschappelijk Onderzoek
ID : ALWGK.2016.025
Informations de copyright
© 2023. The Author(s).
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