The fate of sulfonamide resistance genes and anthropogenic pollution marker
class 1 integron
intI1
one health
river ecosystem
sul1
sul2
sulfamethoxazole
sulfonamide resistance
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2023
2023
Historique:
received:
30
09
2022
accepted:
10
01
2023
entrez:
10
2
2023
pubmed:
11
2
2023
medline:
11
2
2023
Statut:
epublish
Résumé
Currently there are sparse regulations regarding the discharge of antibiotics from wastewater treatment plants (WWTP) into river systems, making surface waters a latent reservoir for antibiotics and antibiotic resistance genes (ARGs). To better understand factors that influence the fate of ARGs in the environment and to foster surveillance of antibiotic resistance spreading in such habitats, several indicator genes have been proposed, including the integrase gene Here we used quantitative PCR and long-read nanopore sequencing to monitor the abundance of these indicator genes and ARGs present as class 1 integron gene cassettes in a river system from pristine source to WWTP-impacted water. ARG abundance was compared with the dynamics of the microbial communities determined Our results show that WWTP effluent was the principal source of all three sulfonamides with highest concentrations for SMX (median 8.6 ng/l), and of the indicator genes We found no evidence that low amounts of sulfonamides in the aquatic environment stimulate the maintenance or even spread of corresponding ARGs. Nevertheless, class 1 integrons carrying various ARGs were still present 13 km downstream from the WWTP. Therefore, limiting the release of ARG-harboring microorganisms may be more crucial for restricting the environmental spread of antimicrobial resistance than attenuating ng/L concentrations of antibiotics.
Identifiants
pubmed: 36760511
doi: 10.3389/fmicb.2023.1058350
pmc: PMC9907086
doi:
Banques de données
figshare
['10.6084/m9.figshare.20310003.v1']
Types de publication
Journal Article
Langues
eng
Pagination
1058350Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2023 Haenelt, Wang, Kasmanas, Musat, Richnow, da Rocha, Müller and Musat.
Déclaration de conflit d'intérêts
HHR is employed by Isodetect Umweltmonitoring GmbH (Leipzig, Germany). The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Nat Rev Microbiol. 2022 May;20(5):257-269
pubmed: 34737424
Nat Methods. 2016 Jul;13(7):581-3
pubmed: 27214047
PLoS One. 2013 Apr 22;8(4):e61217
pubmed: 23630581
Annu Rev Genet. 2010;44:141-66
pubmed: 20707672
Environ Int. 2021 Jan;146:106198
pubmed: 33096465
mBio. 2016 Aug 16;7(4):
pubmed: 27531906
Lancet Planet Health. 2018 Sep;2(9):e398-e405
pubmed: 30177008
Environ Toxicol Chem. 2016 Apr;35(4):767-79
pubmed: 25693841
Nat Commun. 2021 Aug 6;12(1):4765
pubmed: 34362925
Microb Ecol. 2016 Nov;72(4):909-916
pubmed: 27599709
Environ Sci Technol. 2021 May 4;55(9):6087-6096
pubmed: 33852288
Sci Total Environ. 2022 Nov 1;845:157318
pubmed: 35839882
Sci Total Environ. 2020 Jul 20;727:138388
pubmed: 32335446
J Bacteriol. 2008 Jul;190(14):5095-100
pubmed: 18487337
Nucleic Acids Res. 2020 Jan 8;48(D1):D517-D525
pubmed: 31665441
Water Res. 2021 Jun 1;197:117050
pubmed: 33784606
Appl Environ Microbiol. 2020 Apr 17;86(9):
pubmed: 32111585
ISME J. 2014 Apr;8(4):768-77
pubmed: 24152716
Microbiome. 2018 Jul 21;6(1):130
pubmed: 30031405
ISME J. 2016 Feb;10(2):427-36
pubmed: 26394010
Environ Sci Technol. 2017 Nov 21;51(22):13061-13069
pubmed: 28976743
ISME J. 2019 Jul;13(7):1845-1856
pubmed: 30877283
J Hazard Mater. 2019 Feb 5;363:447-456
pubmed: 30342348
Sci Total Environ. 2020 Nov 1;741:140466
pubmed: 32886993
Water Res. 2018 May 15;135:122-133
pubmed: 29466716
Environ Int. 2016 Jan;86:140-9
pubmed: 26590482
Microbiol Spectr. 2017 Sep;5(5):
pubmed: 28944751
Environ Res. 2021 Mar;194:110664
pubmed: 33400949
Nat Ecol Evol. 2022 May;6(5):555-564
pubmed: 35347261
Commun Biol. 2018 Apr 19;1:35
pubmed: 30271921
Nat Rev Microbiol. 2006 Aug;4(8):608-20
pubmed: 16845431
Microbiol Spectr. 2018 Mar;6(2):
pubmed: 29600770
Sci Total Environ. 2021 Sep 15;787:147611
pubmed: 34000537
Water Res. 2021 Aug 1;201:117382
pubmed: 34225233
Environ Int. 2017 Dec;109:155-169
pubmed: 28964562
Environ Int. 2016 Sep;94:736-757
pubmed: 27425630
PLoS One. 2018 Nov 29;13(11):e0208269
pubmed: 30496274
PLoS Comput Biol. 2021 Nov 8;17(11):e1009581
pubmed: 34748542
Sci Total Environ. 2021 Jan 10;751:142217
pubmed: 33181985
Nat Rev Microbiol. 2015 May;13(5):310-7
pubmed: 25817583
ISME J. 2015 Jun;9(6):1269-79
pubmed: 25500508
Microbiome. 2018 May 17;6(1):90
pubmed: 29773078
Isotopes Environ Health Stud. 2018 May;54(2):168-184
pubmed: 29436855
Science. 2009 May 22;324(5930):1034
pubmed: 19460999
Int J Antimicrob Agents. 2012 Sep;40(3):264-7
pubmed: 22817917
Sci Total Environ. 2017 Dec 1;601-602:1849-1868
pubmed: 28629112
Environ Pollut. 2021 May 1;276:116733
pubmed: 33631686
Environ Sci Technol. 2006 Dec 1;40(23):7445-50
pubmed: 17181002
Environ Sci Technol. 2016 Oct 18;50(20):11346-11356
pubmed: 27643810
Bioinformatics. 2009 Apr 15;25(8):1096-8
pubmed: 19228805
Environ Sci Technol. 2010 Oct 1;44(19):7220-5
pubmed: 20509603
PLoS One. 2020 Mar 4;15(3):e0229451
pubmed: 32130234
Sci Total Environ. 2021 Nov 20;796:148919
pubmed: 34273824
Nat Biotechnol. 2019 Aug;37(8):852-857
pubmed: 31341288
Environ Sci Pollut Res Int. 2019 Jun;26(18):18930-18937
pubmed: 31055743