Barrier properties of Nup98 FG phases ruled by FG motif identity and inter-FG spacer length.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
10 02 2023
10 02 2023
Historique:
received:
14
10
2022
accepted:
24
01
2023
pubmed:
11
2
2023
medline:
15
2
2023
entrez:
10
2
2023
Statut:
epublish
Résumé
Nup98 FG repeat domains comprise hydrophobic FG motifs linked through uncharged spacers. FG motifs capture nuclear transport receptors (NTRs) during nuclear pore complex (NPC) passage, confer inter-repeat cohesion, and condense the domains into a selective phase with NPC-typical barrier properties. We show that shortening inter-FG spacers enhances cohesion, increases phase density, and tightens such barrier - all consistent with a sieve-like phase. Phase separation tolerates mutating the Nup98-typical GLFG motifs, provided domain-hydrophobicity remains preserved. NTR-entry, however, is sensitive to (certain) deviations from canonical FG motifs, suggesting co-evolutionary adaptation. Unexpectedly, we observed that arginines promote FG-phase-entry apparently also by hydrophobic interactions/ hydrogen-bonding and not just through cation-π interactions. Although incompatible with NTR·cargo complexes, a YG phase displays remarkable transport selectivity, particularly for engineered GFP
Identifiants
pubmed: 36765044
doi: 10.1038/s41467-023-36331-4
pii: 10.1038/s41467-023-36331-4
pmc: PMC9918544
doi:
Substances chimiques
Nuclear Pore Complex Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
747Informations de copyright
© 2023. The Author(s).
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