Immune Cells Release MicroRNA-155 Enriched Extracellular Vesicles That Promote HIV-1 Infection.
HIV-1
TRL7/8
calprotectin
extracellular vesicles
immune activation
inflammation
miR-155
micro-RNA
pathogenesis
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
31 01 2023
31 01 2023
Historique:
received:
26
11
2022
revised:
21
01
2023
accepted:
25
01
2023
entrez:
11
2
2023
pubmed:
12
2
2023
medline:
15
2
2023
Statut:
epublish
Résumé
The hallmark of HIV-1 infection is the rapid dysregulation of immune functions. Recent investigations for biomarkers of such dysregulation in people living with HIV (PLWH) reveal a strong correlation between viral rebound and immune activation with an increased abundance of extracellular vesicles (EVs) enriched with microRNA-155. We propose that the activation of peripheral blood mononuclear cells (PBMCs) leads to an increased miR-155 expression and production of miR-155-rich extracellular vesicles (miR-155-rich EVs), which can exacerbate HIV-1 infection by promoting viral replication. PBMCs were incubated with either HIV-1 (NL4.3Balenv), a TLR-7/8 agonist, or TNF. EVs were harvested from the cell culture supernatant by differential centrifugation, and RT-qPCR quantified miR-155 in cells and derived EVs. The effect of miR-155-rich EVs on replication of HIV-1 in incubated PBMCs was then measured by viral RNA and DNA quantification. HIV-1, TLR7/8 agonist, and TNF each induced the release of miR-155-rich EVs by PBMCs. These miR-155-rich EVs increased viral replication in PBMCs infected in vitro. Infection with HIV-1 and inflammation promote the production of miR-155-rich EVs, enhancing viral replication. Such autocrine loops, therefore, could influence the course of HIV-1 infection by promoting viral replication.
Identifiants
pubmed: 36766808
pii: cells12030466
doi: 10.3390/cells12030466
pmc: PMC9914104
pii:
doi:
Substances chimiques
MicroRNAs
0
MIRN155 microRNA, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : CIHR
ID : MOP-391232
Pays : Canada
Organisme : CIHR
ID : MOP-188726
Pays : Canada
Organisme : CIHR
ID : MOP-267056
Pays : Canada
Références
PLoS One. 2014 Jun 30;9(6):e100820
pubmed: 24978053
J Virol. 2009 Jan;83(2):512-21
pubmed: 18987139
Virology. 1999 Jan 5;253(1):10-6
pubmed: 9887314
J Virol. 2014 Oct;88(19):11529-39
pubmed: 25056899
J Clin Microbiol. 2005 Jun;43(6):2709-17
pubmed: 15956387
Cell Mol Neurobiol. 2016 Apr;36(3):301-12
pubmed: 27053351
Cell Microbiol. 2013 Mar;15(3):412-29
pubmed: 23072732
J Immunol. 1989 Jan 15;142(2):431-8
pubmed: 2463307
Pathogens. 2021 Apr 27;10(5):
pubmed: 33925397
Mucosal Immunol. 2016 Jan;9(1):194-205
pubmed: 26104913
J Virol. 1997 Mar;71(3):1922-30
pubmed: 9032323
Traffic. 2010 Jan;11(1):110-22
pubmed: 19912576
Nat Commun. 2020 Jan 10;11(1):214
pubmed: 31924781
J Mol Biol. 2013 Oct 9;425(19):3582-600
pubmed: 23500488
J Extracell Vesicles. 2012 Apr 16;1:
pubmed: 24009886
C R Biol. 2017 Feb;340(2):114-131
pubmed: 28081967
Mol Med Rep. 2017 Aug;16(2):1584-1589
pubmed: 28627655
Eur J Immunol. 2011 Apr;41(4):1058-69
pubmed: 21337543
PLoS One. 2016 Nov 23;11(11):e0167091
pubmed: 27880829
Cells. 2022 Mar 02;11(5):
pubmed: 35269481
Viruses. 2019 Dec 19;12(1):
pubmed: 31861621
Viruses. 2021 Nov 12;13(11):
pubmed: 34835072
Proc Natl Acad Sci U S A. 2007 Feb 20;104(8):2750-5
pubmed: 17293455
BMC Res Notes. 2017 Aug 14;10(1):403
pubmed: 28807007
J Immunol. 2006 Mar 1;176(5):2888-95
pubmed: 16493046
Proc Natl Acad Sci U S A. 2018 Oct 9;115(41):E9659-E9667
pubmed: 30257946
Front Cell Infect Microbiol. 2012 Jun 01;2:73
pubmed: 22919664
J Biol Chem. 2013 Jul 5;288(27):20014-33
pubmed: 23661700
Arthritis Res Ther. 2011 Apr 14;13(2):R60
pubmed: 21492422
Nat Commun. 2015 Jun 18;6:7321
pubmed: 26084661
J Virol. 2002 Feb;76(3):1004-14
pubmed: 11773376
Mediators Inflamm. 2016;2016:8060182
pubmed: 27843203
Gene. 2013 Dec 10;532(1):1-12
pubmed: 23246696
J Immunol. 2003 Mar 15;170(6):3233-42
pubmed: 12626582
J Antimicrob Chemother. 2014 Nov;69(11):3041-6
pubmed: 25011654
Science. 2001 Oct 26;294(5543):853-8
pubmed: 11679670
J Immunol. 2003 Sep 1;171(5):2435-45
pubmed: 12928391
Blood. 2003 Feb 1;101(3):1024-9
pubmed: 12393488
Adv Exp Med Biol. 2018;1075:213-239
pubmed: 30030795
Microbes Infect. 2012 Mar;14(3):247-61
pubmed: 22064066
Br J Cancer. 2010 Feb 16;102(4):639-44
pubmed: 20087353
J Clin Invest. 2012 Nov;122(11):4190-202
pubmed: 23041630
Free Radic Biol Med. 2017 Mar;104:185-198
pubmed: 28087411
HIV Med. 2017 May;18(5):354-362
pubmed: 27981723
Oncogene. 2016 Nov 3;35(44):5735-5745
pubmed: 27086923
Retrovirology. 2018 Oct 23;15(1):71
pubmed: 30352600
J Clin Invest. 2019 Feb 1;129(2):712-726
pubmed: 30640176
Cold Spring Harb Protoc. 2010 Jun;2010(6):pdb.prot5439
pubmed: 20516177
Pathog Immun. 2021 Jan 14;6(1):1-28
pubmed: 33987483
Cold Spring Harb Perspect Biol. 2018 Jul 2;10(7):
pubmed: 28716890
Front Immunol. 2021 Jan 18;11:617522
pubmed: 33537035
Proc Natl Acad Sci U S A. 1989 Apr;86(7):2365-8
pubmed: 2784570
Nat Cell Biol. 2007 Jun;9(6):654-9
pubmed: 17486113
J Leukoc Biol. 2004 Aug;76(2):433-40
pubmed: 15107458
AIDS. 2014 Jan 14;28(2):171-80
pubmed: 24413309
Eur J Immunol. 2008 Feb;38(2):400-9
pubmed: 18228247
Blood. 2008 Jan 15;111(2):699-704
pubmed: 17909079
J Infect Dis. 2015 Jun 1;211(11):1712-6
pubmed: 25512626
Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):20047-52
pubmed: 23169640
Cell. 2007 Jun 29;129(7):1401-14
pubmed: 17604727
Leukemia. 2014 Dec;28(12):2324-35
pubmed: 24727676
Mol Cell Proteomics. 2019 Mar;18(3):461-476
pubmed: 30504243
Sci Rep. 2018 May 8;8(1):7227
pubmed: 29740045
Mol Biol Rep. 2022 Aug;49(8):7783-7792
pubmed: 35733067
Front Immunol. 2022 Aug 29;13:916599
pubmed: 36105810
J Acquir Immune Defic Syndr. 2015 Nov 1;70(3):219-27
pubmed: 26181817
Virol J. 2007 Dec 03;4:130
pubmed: 18053162
J Biol Chem. 2015 May 29;290(22):13736-48
pubmed: 25873391
AIDS Res Hum Retroviruses. 2011 Feb;27(2):167-78
pubmed: 20964480
J Virol. 2018 May 29;92(12):
pubmed: 29593039
J Interferon Cytokine Res. 2019 Jun;39(6):321-330
pubmed: 30998423
Biol Chem. 2018 May 24;399(6):525-547
pubmed: 29447113
J Immunol Res. 2015;2015:296149
pubmed: 27057553
J Biol Chem. 2017 Dec 15;292(50):20683-20693
pubmed: 29066622
Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7113-8
pubmed: 19359473
PLoS Genet. 2021 Dec 6;17(12):e1009951
pubmed: 34871319
Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12481-6
pubmed: 16885212
Sci Rep. 2017 May 10;7(1):1695
pubmed: 28490736
J Immunol. 2012 Jul 15;189(2):744-54
pubmed: 22711894
Int J Biol Sci. 2019 Nov 15;15(13):2925-2935
pubmed: 31853228
Nucleic Acids Res. 2012 Apr;40(8):3548-62
pubmed: 22187158
J Virol. 2014 Nov;88(21):12385-96
pubmed: 25122785
J Immunol Methods. 2003 Sep;280(1-2):1-11
pubmed: 12972183