Self-Assembling Enzymatic Nanocomplexes with Polypeptides and Low-Weight Organic Compounds: Preparation, Characterization, and Application of New Antibacterials.
antibacterials
emodin
enzyme
hexahistidine-tagged organophosphorus hydrolase
molecular modeling
nanomaterial
penicillin acylase
polymyxins
quorum quenching
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
17 Jan 2023
17 Jan 2023
Historique:
received:
20
12
2022
revised:
12
01
2023
accepted:
15
01
2023
entrez:
11
2
2023
pubmed:
12
2
2023
medline:
15
2
2023
Statut:
epublish
Résumé
The self-assembling of nanosized materials is a promising field for research and development. Multiple approaches are applied to obtain inorganic, organic and composite nanomaterials with different functionality. In the present work, self-assembling nanocomplexes (NCs) were prepared on the basis of enzymes and polypeptides followed by the investigation of the influence of low-molecular weight biologically active compounds on the properties of the NCs. For that, the initially possible formation of catalytically active self-assembling NCs of four hydrolytic enzymes with nine effectors was screened via molecular modeling. It allowed the selection of two enzymes (hexahistidine-tagged organophosphorus hydrolase and penicillin acylase) and two compounds (emodin and naringenin) having biological activity. Further, such NCs based on surface-modified enzymes were characterized by a batch of physical and biochemical methods. At least three NCs containing emodin and enzyme (His
Identifiants
pubmed: 36768158
pii: ijms24031831
doi: 10.3390/ijms24031831
pmc: PMC9915939
pii:
doi:
Substances chimiques
Penicillin Amidase
EC 3.5.1.11
Emodin
KA46RNI6HN
Anti-Bacterial Agents
0
Peptides
0
Aryldialkylphosphatase
EC 3.1.8.1
Organic Chemicals
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : State Task of Lomonosov Moscow State University
ID : 121041500039-8
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