Degraded RNA from Human Anterior Cruciate Ligaments Yields Valid Gene Expression Profiles.
RNA
biomechanics
gene expression
knee
ligament
tendon
tissue remodeling
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
18 Jan 2023
18 Jan 2023
Historique:
received:
19
12
2022
revised:
12
01
2023
accepted:
13
01
2023
entrez:
11
2
2023
pubmed:
12
2
2023
medline:
15
2
2023
Statut:
epublish
Résumé
Correlating gene expression patterns with biomechanical properties of connective tissues provides insights into the molecular processes underlying the tissue growth and repair. Cadaveric specimens such as human knees are widely considered suitable for biomechanical studies, but their usefulness for gene expression experiments is potentially limited by the unavoidable, nuclease-mediated degradation of RNA. Here, we tested whether valid gene expression profiles can be obtained using degraded RNA from human anterior cruciate ligaments (ACLs). Human ACL RNA (N = 6) degraded in vitro by limited ribonuclease digestion resemble highly degraded RNA isolated from cadaveric tissue. PCR threshold cycle (C
Identifiants
pubmed: 36768233
pii: ijms24031895
doi: 10.3390/ijms24031895
pmc: PMC9916516
pii:
doi:
Substances chimiques
RNA
63231-63-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAMS NIH HHS
ID : AR049767
Pays : United States
Organisme : TTU/TTUHSC Clinical/Basic Research Grant Program
ID : none
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