Closing the Gap between the Auditory Nerve and Cochlear Implant Electrodes: Which Neurotrophin Cocktail Performs Best for Axonal Outgrowth and Is Electrical Stimulation Beneficial?
cochlear implant
electrical stimulation
nerve regeneration
neurotrophin treatment
spiral ganglion
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
19 Jan 2023
19 Jan 2023
Historique:
received:
29
11
2022
revised:
12
01
2023
accepted:
14
01
2023
entrez:
11
2
2023
pubmed:
12
2
2023
medline:
15
2
2023
Statut:
epublish
Résumé
Neurotrophins promote neurite outgrowth of auditory neurons and may help closing the gap to cochlear implant (CI) electrodes to enhance electrical hearing. The best concentrations and mix of neurotrophins for this nerve regrowth are unknown. Whether electrical stimulation (ES) during outgrowth is beneficial or may direct axons is another open question. Auditory neuron explant cultures of distinct cochlear turns of 6-7 days old mice were cultured for four days. We tested different concentrations and combinations of BDNF and NT-3 and quantified the numbers and lengths of neurites with an advanced automated analysis. A custom-made 24-well electrical stimulator based on two bulk CIs served to test different ES strategies. Quantification of receptors trkB, trkC, p75
Identifiants
pubmed: 36768339
pii: ijms24032013
doi: 10.3390/ijms24032013
pmc: PMC9916558
pii:
doi:
Substances chimiques
Brain-Derived Neurotrophic Factor
0
Receptors, Nerve Growth Factor
0
Neurotrophin 3
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : FWF Austrian Science Fund
ID : I 3154 Gapless Man: Machine Interface
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