Yet Another Similarity between Mitochondrial and Bacterial Ribosomal Small Subunit Biogenesis Obtained by Structural Characterization of RbfA from


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
20 Jan 2023
Historique:
received: 28 12 2022
revised: 15 01 2023
accepted: 18 01 2023
entrez: 11 2 2023
pubmed: 12 2 2023
medline: 15 2 2023
Statut: epublish

Résumé

Ribosome biogenesis is a complex and highly accurate conservative process of ribosomal subunit maturation followed by association. Subunit maturation comprises sequential stages of ribosomal RNA and proteins' folding, modification and binding, with the involvement of numerous RNAses, helicases, GTPases, chaperones, RNA, protein-modifying enzymes, and assembly factors. One such assembly factor involved in bacterial 30S subunit maturation is ribosomal binding factor A (RbfA). In this study, we present the crystal (determined at 2.2 Å resolution) and NMR structures of RbfA as well as the 2.9 Å resolution cryo-EM reconstruction of the 30S-RbfA complex from

Identifiants

pubmed: 36768442
pii: ijms24032118
doi: 10.3390/ijms24032118
pmc: PMC9917171
pii:
doi:

Substances chimiques

Ribosomal Proteins 0
Escherichia coli Proteins 0
RNA, Ribosomal, 16S 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Russian Foundation for Basic Research
ID : 20-54-15001

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Auteurs

Aydar G Bikmullin (AG)

Laboratory of Structural Biology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420021 Kazan, Russia.

Bulat Fatkhullin (B)

Department of Integrated Structural Biology, Institute of Genetics and Molecular and Cellular Biology, University of Strasbourg, 67400 Illkirch, France.
Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Russia.

Artem Stetsenko (A)

Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, 9700 AB Groningen, The Netherlands.

Azat Gabdulkhakov (A)

Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Russia.

Natalia Garaeva (N)

Laboratory of Structural Biology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420021 Kazan, Russia.

Liliia Nurullina (L)

Department of Integrated Structural Biology, Institute of Genetics and Molecular and Cellular Biology, University of Strasbourg, 67400 Illkirch, France.

Evelina Klochkova (E)

Laboratory of Structural Biology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420021 Kazan, Russia.

Alexander Golubev (A)

Laboratory of Structural Biology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420021 Kazan, Russia.

Iskander Khusainov (I)

Open Lab 'Biomarker', Kazan Federal University, 420008 Kazan, Russia.

Natalie Trachtmann (N)

Institute of Microbiology, University of Stuttgart, D-70569 Stuttgart, Germany.

Dmitriy Blokhin (D)

NMR Laboratory, Medical Physics Department, Institute of Physics, Kazan Federal University, 420008 Kazan, Russia.

Albert Guskov (A)

Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, 9700 AB Groningen, The Netherlands.

Shamil Validov (S)

Laboratory of Structural Biology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420021 Kazan, Russia.
Federal Research Center "Kazan Scientific Center of Russian Academy of Sciences", 420111 Kazan, Russia.

Konstantin Usachev (K)

Laboratory of Structural Biology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420021 Kazan, Russia.
Federal Research Center "Kazan Scientific Center of Russian Academy of Sciences", 420111 Kazan, Russia.

Marat Yusupov (M)

Laboratory of Structural Biology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420021 Kazan, Russia.
Department of Integrated Structural Biology, Institute of Genetics and Molecular and Cellular Biology, University of Strasbourg, 67400 Illkirch, France.

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Classifications MeSH