RNA Extraction from Cartilage: Issues, Methods, Tips.
RNA
cartilage
extraction methods
fresh tissue
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
20 Jan 2023
20 Jan 2023
Historique:
received:
24
11
2022
revised:
07
01
2023
accepted:
18
01
2023
entrez:
11
2
2023
pubmed:
12
2
2023
medline:
15
2
2023
Statut:
epublish
Résumé
The increase in degenerative diseases involving articular cartilage has pushed research to focus on their pathogenesis and treatment, exploiting increasingly complex techniques. Gene expression analyses from tissue are representative of the in vivo situation, but the protocols to be applied to obtain a reliable analysis are not completely cleared through customs. Thus, RNA extraction from fresh samples and specifically from musculoskeletal tissue such as cartilage is still a challenging issue. The aim of the review is to provide an overview of the techniques described in the literature for RNA extraction, highlighting limits and possibilities. The research retrieved 65 papers suitable for the purposes. The results highlighted the great difficulty in comparing the different studies, both for the sources of tissue used and for the techniques employed, as well as the details about protocols. Few papers compared different RNA extraction methods or homogenization techniques; the case study reported by authors about RNA extraction from sheep cartilage has not found an analog in the literature, confirming the existence of a relevant blank on studies about RNA extraction from cartilage tissue. However, the state of the art depicted can be used as a starting point to improve and expand studies on this topic.
Identifiants
pubmed: 36768444
pii: ijms24032120
doi: 10.3390/ijms24032120
pmc: PMC9917073
pii:
doi:
Substances chimiques
RNA
63231-63-0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministry of Health
ID : Ricerca Corrente
Références
Biotechniques. 1995 Sep;19(3):340-4
pubmed: 7495539
J Biotechnol. 2007 Jan 20;127(4):549-59
pubmed: 16945445
J Cell Biochem. 1996 Dec 15;63(4):395-409
pubmed: 8978456
Anal Biochem. 2017 Feb 1;518:134-138
pubmed: 27913164
J Biol Chem. 1996 Aug 2;271(31):18954-60
pubmed: 8702559
J Cell Physiol. 2018 Jun;233(6):4401-4407
pubmed: 29206301
Methods Mol Med. 2004;100:101-4
pubmed: 15280590
Osteoarthritis Cartilage. 2005 Jun;13(6):508-18
pubmed: 15922185
Histochem Cell Biol. 2005 Dec;124(6):535-45
pubmed: 16133125
J Biol Chem. 1993 Jan 15;268(2):1440-7
pubmed: 7678258
Biotechniques. 1993 Sep;15(3):532-4, 536-7
pubmed: 7692896
Anal Biochem. 1992 Jun;203(2):352-6
pubmed: 1384388
Biomaterials. 2021 May;272:120773
pubmed: 33798958
Anal Biochem. 2002 Jan 1;300(1):1-10
pubmed: 11743684
Adv Med Sci. 2008;53(2):191-7
pubmed: 18467268
J Orthop Res. 2017 Mar;35(3):580-589
pubmed: 27411036
Clin Exp Rheumatol. 1994 Mar-Apr;12(2):169-73
pubmed: 7518759
Scand J Rheumatol. 1998;27(1):61-7
pubmed: 9506880
Mater Sci Eng C Mater Biol Appl. 2017 Jan 1;70(Pt 1):101-111
pubmed: 27770869
Am J Clin Pathol. 2002 Nov;118(5):733-41
pubmed: 12428794
Histochem J. 2000 Mar;32(3):175-86
pubmed: 10841312
J Cell Biol. 1989 Oct;109(4 Pt 1):1849-56
pubmed: 2477383
Osteoarthritis Cartilage. 2013 Mar;21(3):450-61
pubmed: 23220557
Methods Mol Med. 2004;100:109-28
pubmed: 15280592
Head Neck Oncol. 2010 Oct 07;2:27
pubmed: 20929582
Bone Joint Res. 2016 Nov;5(11):560-568
pubmed: 27881439
Anal Biochem. 2000 Nov 1;286(1):80-5
pubmed: 11038277
Biochem Biophys Res Commun. 1993 May 14;192(3):1169-74
pubmed: 8507190
J Rheumatol. 2006 Jul;33(7):1329-40
pubmed: 16821268
Biotechniques. 1997 Jun;22(6):1082-6
pubmed: 9187757
Methods Mol Biol. 2011;675:405-13
pubmed: 20949406
Am J Vet Res. 2000 Aug;61(8):900-5
pubmed: 10951979
Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8254-8
pubmed: 7690145
Biochem Soc Trans. 2002 Nov;30(Pt 6):879-82
pubmed: 12440937
Rheumatol Int. 2008 Mar;28(5):437-43
pubmed: 17924112
Nucleic Acids Res. 2005 Mar 30;33(6):e56
pubmed: 15800207
J Orthop Surg Res. 2017 Mar 28;12(1):51
pubmed: 28351380
Arch Oral Biol. 1998 Jan;43(1):83-92
pubmed: 9569994
J Orthop Res. 2001 May;19(3):478-81
pubmed: 11398863
Genet Mol Res. 2016 Jun 03;15(2):
pubmed: 27323116
Anal Biochem. 1989 Dec;183(2):301-4
pubmed: 2483036
Anal Biochem. 1992 Apr;202(1):89-95
pubmed: 1621990
Arthritis Rheum. 2001 Mar;44(3):578-84
pubmed: 11263772
J Clin Pathol. 2001 Oct;54(10):778-82
pubmed: 11577126
Nat Rev Rheumatol. 2016 Nov;12(11):632-644
pubmed: 27652499
Anal Biochem. 1995 Mar 1;225(2):356-60
pubmed: 7762805
J Orthop Res. 2006 Aug;24(8):1765-72
pubmed: 16779831
Hear Res. 2015 Jul;325:42-8
pubmed: 25818515
Nucleic Acids Res. 1986 Nov 11;14(21):8347-60
pubmed: 3537957
Lab Invest. 2006 Feb;86(2):202-11
pubmed: 16402036
Cartilage. 2021 Apr;12(2):222-225
pubmed: 30841716
Am J Vet Res. 2006 Aug;67(8):1438-44
pubmed: 16881859
Tissue Eng Part B Rev. 2013 Aug;19(4):353-67
pubmed: 23339690
Biochem Biophys Res Commun. 1998 Oct 29;251(3):824-9
pubmed: 9790994
Osteoarthritis Cartilage. 2009 Mar;17(3):328-35
pubmed: 18775662
J Bone Miner Res. 2002 Feb;17(2):275-83
pubmed: 11811558
Matrix Biol. 2004 Jul;23(4):219-30
pubmed: 15296936
Arthritis Rheum. 1992 Oct;35(10):1227-33
pubmed: 1418008
Biochem Biophys Res Commun. 2003 Dec 5;312(1):131-7
pubmed: 14630030
Biotechniques. 2004 Apr;36(4):628-33
pubmed: 15088381
Nucleic Acids Res. 2011 May;39(9):e63
pubmed: 21317187
J Clin Med. 2021 Apr 28;10(9):
pubmed: 33925222
J Prosthet Dent. 1997 Apr;77(4):435-43
pubmed: 9104721
J Bone Miner Res. 1999 Feb;14(2):304-10
pubmed: 9933486
Mol Aspects Med. 2006 Apr-Jun;27(2-3):126-39
pubmed: 16469371
Methods Mol Biol. 2021;2245:121-133
pubmed: 33315199
Sci Rep. 2021 Jan 15;11(1):1626
pubmed: 33452305
Osteoarthritis Cartilage. 2011 Apr;19(4):458-65
pubmed: 21262368
Methods Mol Biol. 2007;362:315-27
pubmed: 17417019
Osteoarthritis Cartilage. 2005 Feb;13(2):162-70
pubmed: 15694578
Biotechniques. 1996 Jun;20(6):968-70
pubmed: 8780864
Connect Tissue Res. 1994;30(3):203-14
pubmed: 8039387
J Rheumatol Suppl. 1991 Feb;27:55-7
pubmed: 2027132
Biochemistry. 1979 Nov 27;18(24):5294-9
pubmed: 518835
Int J Mol Sci. 2021 Jan 15;22(2):
pubmed: 33467447
J Nippon Med Sch. 2018;85(6):309-314
pubmed: 30568056
Biochim Biophys Acta. 1998 Feb 2;1379(2):171-7
pubmed: 9528651
Anal Biochem. 2012 Oct 1;429(1):39-41
pubmed: 22776092
Connect Tissue Res. 2015 Jun;56(3):236-43
pubmed: 25738598
Arthritis Rheum. 2010 Apr;62(4):1108-18
pubmed: 20131271
Sci Rep. 2019 Sep 19;9(1):13603
pubmed: 31537813
BMC Res Notes. 2010 Jan 18;3:7
pubmed: 20180968
Am J Vet Res. 2001 Jul;62(7):1088-94
pubmed: 11453485
J Cell Biol. 1990 Apr;110(4):1369-78
pubmed: 1691190
MethodsX. 2017 Oct 25;4:423-428
pubmed: 29124019
Matrix Biol. 1997 Apr;16(1):1-11
pubmed: 9181549
BMC Mol Biol. 2006 Jan 31;7:3
pubmed: 16448564
Anal Biochem. 1987 Apr;162(1):156-9
pubmed: 2440339